2qn4
From Proteopedia
(Difference between revisions)
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<StructureSection load='2qn4' size='340' side='right'caption='[[2qn4]], [[Resolution|resolution]] 1.80Å' scene=''> | <StructureSection load='2qn4' size='340' side='right'caption='[[2qn4]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[2qn4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/ | + | <table><tr><td colspan='2'>[[2qn4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QN4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QN4 FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qn4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qn4 OCA], [https://pdbe.org/2qn4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qn4 RCSB], [https://www.ebi.ac.uk/pdbsum/2qn4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qn4 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qn4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qn4 OCA], [https://pdbe.org/2qn4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qn4 RCSB], [https://www.ebi.ac.uk/pdbsum/2qn4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qn4 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | + | [https://www.uniprot.org/uniprot/IAAS_ORYSJ IAAS_ORYSJ] This protein inhibits independently subtilisin and T.castaneum alpha-amylase but not barley alpha-amylase. | |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qn4 ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qn4 ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Rice bifunctional alpha-amylase/subtilisin inhibitor (RASI) can inhibit both alpha-amylase from larvae of the red flour beetle (Tribolium castaneum) and subtilisin from Bacillus subtilis. The synthesis of RASI is up-regulated during the late milky stage in developing seeds. The 8.9 kDa molecular-weight RASI from rice has been crystallized using the hanging-drop vapour-diffusion method. According to 1.81 angstroms resolution X-ray diffraction data from rice RASI crystals, the crystal belongs to space group P2(1)2(1)2, with unit-cell parameters a = 79.99, b = 62.95, c = 66.70 angstroms. Preliminary analysis indicates two RASI molecules in an asymmetric unit with a solvent content of 44%. | ||
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| - | Purification, crystallization and preliminary X-ray crystallographic analysis of rice bifunctional alpha-amylase/subtilisin inhibitor from Oryza sativa.,Lin YH, Peng WY, Huang YC, Guan HH, Hsieh YC, Liu MY, Chang T, Chen CJ Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Aug 1;62(Pt, 8):743-5. Epub 2006 Jul 24. PMID:16880545<ref>PMID:16880545</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 2qn4" style="background-color:#fffaf0;"></div> | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Oryza sativa | + | [[Category: Oryza sativa Japonica Group]] |
| - | [[Category: Chen | + | [[Category: Chen CJ]] |
| - | [[Category: Guan | + | [[Category: Guan HH]] |
| - | [[Category: Hsieh | + | [[Category: Hsieh YC]] |
| - | [[Category: Huang | + | [[Category: Huang YC]] |
| - | [[Category: Lin | + | [[Category: Lin YH]] |
| - | [[Category: Peng | + | [[Category: Peng WY]] |
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Revision as of 09:01, 25 October 2023
Structure and function study of rice bifunctional alpha-amylase/subtilisin inhibitor from Oryza sativa
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