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2rdh

From Proteopedia

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Crystal structure of Staphylococcal Superantigen-Like protein 11

Structural highlights

2rdh is a 4 chain structure with sequence from Staphylococcus aureus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.7Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SLL11_STAAU Secreted protein that plays a role in the inhibition of host immune system. Targets myeloid cells such as monocytes or granulocytes through binding with sialyllactosamine-containing glycoproteins (PubMed:18045383). Prevents initial rolling of neutrophils toward the site of infection by interacting with host SELPLG (PubMed:18045383). Disrupts neutrophil motility by induction of cell adhesion via interacting with glycans but independently of SELPLG (PubMed:30862940).^[1] ^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Staphylococcus aureus is a major pathogen that produces a family of 14 staphylococcal superantigen-like (SSL) proteins, which are structurally similar to superantigens but do not stimulate T cells. SSL11 is one member of the family that is found in all staphylococcal strains. Recombinant SSL11 bound to granulocytes and monocytes through a sialic acid-dependent mechanism and was rapidly internalized. SSL11 also bound to sialic acid-containing glycoproteins, such as the Fc receptor for IgA (FcalphaRI) and P-selectin glycoprotein ligand-1 (PSGL-1), and inhibited neutrophil attachment to a P-selectin-coated surface. Biosensor analysis of two SSL11 alleles binding to sialyl Lewis X [sLe(x)- Neu5Acalpha2-3Galbeta1-4(Fuc1-3)GlcNAc] coupled to bovine serum albumin gave dissociation constants of 0.7 and 7 mum respectively. Binding of SSL11 to a glycan array revealed specificity for glycans containing the trisaccharide sialyllactosamine (sLacNac - Neu5Acalpha2-3Galbeta1-4GlcNAc). A 1.6 A resolution crystal structure of SSL11 complexed with sLe(x) revealed a discrete binding site in the C-terminal beta-grasp domain, with predominant interactions with the sialic acid and galactose residues. A single amino acid mutation in the carbohydrate binding site abolished all SSL11 binding. Thus, SSL11 is a staphylococcal protein that targets myeloid cells by binding sialyllactosamine-containing glycoproteins.

The crystal structure of staphylococcal superantigen-like protein 11 in complex with sialyl Lewis X reveals the mechanism for cell binding and immune inhibition.,Chung MC, Wines BD, Baker H, Langley RJ, Baker EN, Fraser JD Mol Microbiol. 2007 Dec;66(6):1342-55. PMID:18045383^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chung MC, Wines BD, Baker H, Langley RJ, Baker EN, Fraser JD. The crystal structure of staphylococcal superantigen-like protein 11 in complex with sialyl Lewis X reveals the mechanism for cell binding and immune inhibition. Mol Microbiol. 2007 Dec;66(6):1342-55. PMID:18045383 doi:10.1111/j.1365-2958.2007.05989.x
↑ Chen C, Yang C, Barbieri JT. Staphylococcal Superantigen-like protein 11 mediates neutrophil adhesion and motility arrest, a unique bacterial toxin action. Sci Rep. 2019 Mar 12;9(1):4211. PMID:30862940 doi:10.1038/s41598-019-40817-x
↑ Chung MC, Wines BD, Baker H, Langley RJ, Baker EN, Fraser JD. The crystal structure of staphylococcal superantigen-like protein 11 in complex with sialyl Lewis X reveals the mechanism for cell binding and immune inhibition. Mol Microbiol. 2007 Dec;66(6):1342-55. PMID:18045383 doi:10.1111/j.1365-2958.2007.05989.x

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2rdh"

@@ Line 3: / Line 3: @@
 <StructureSection load='2rdh' size='340' side='right'caption='[[2rdh]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2rdh]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RDH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RDH FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2rdh]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RDH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RDH FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2rdg|2rdg]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SSL11 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rdh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rdh OCA], [https://pdbe.org/2rdh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rdh RCSB], [https://www.ebi.ac.uk/pdbsum/2rdh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rdh ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/SLL11_STAAU SLL11_STAAU] Secreted protein that plays a role in the inhibition of host immune system. Targets myeloid cells such as monocytes or granulocytes through binding with sialyllactosamine-containing glycoproteins (PubMed:18045383). Prevents initial rolling of neutrophils toward the site of infection by interacting with host SELPLG (PubMed:18045383). Disrupts neutrophil motility by induction of cell adhesion via interacting with glycans but independently of SELPLG (PubMed:30862940).<ref>PMID:18045383</ref> <ref>PMID:30862940</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 33: / Line 34: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Baker, E N]]
+[[Category: Staphylococcus aureus]]
-[[Category: Baker, H]]
+[[Category: Baker EN]]
-[[Category: Chung, M C]]
+[[Category: Baker H]]
-[[Category: Fraser, J D]]
+[[Category: Chung MC]]
-[[Category: Langley, R J]]
+[[Category: Fraser JD]]
-[[Category: Wines, B D]]
+[[Category: Langley RJ]]
-[[Category: Beta grasp]]
+[[Category: Wines BD]]
-[[Category: Ob fold]]
-[[Category: Toxin]]

2rdh

From Proteopedia

Current revision

Crystal structure of Staphylococcal Superantigen-Like protein 11

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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