1nto

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1nto.gif|left|200px]]
[[Image:1nto.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1nto |SIZE=350|CAPTION= <scene name='initialview01'>1nto</scene>, resolution 1.94&Aring;
+
The line below this paragraph, containing "STRUCTURE_1nto", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Alcohol_dehydrogenase Alcohol dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.1 1.1.1.1] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE= ADH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 Sulfolobus solfataricus])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1nto| PDB=1nto | SCENE= }}
-
|RELATEDENTRY=[[1jvb|1JVB]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nto FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nto OCA], [http://www.ebi.ac.uk/pdbsum/1nto PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1nto RCSB]</span>
+
-
}}
+
'''N249Y MUTANT OF ALCOHOL DEHYDROGENASE FROM THE ARCHAEON SULFOLOBUS SOLFATARICUS-MONOCLINIC CRYSTAL FORM'''
'''N249Y MUTANT OF ALCOHOL DEHYDROGENASE FROM THE ARCHAEON SULFOLOBUS SOLFATARICUS-MONOCLINIC CRYSTAL FORM'''
Line 34: Line 31:
[[Category: Sica, F.]]
[[Category: Sica, F.]]
[[Category: Zagari, A.]]
[[Category: Zagari, A.]]
-
[[Category: archaeon]]
+
[[Category: Archaeon]]
-
[[Category: mutant]]
+
[[Category: Mutant]]
-
[[Category: nad(h)-dependent]]
+
[[Category: Zinc]]
-
[[Category: zinc]]
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:58:01 2008''
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:35:23 2008''
+

Revision as of 23:58, 2 May 2008

Image:1nto.gif

Template:STRUCTURE 1nto

N249Y MUTANT OF ALCOHOL DEHYDROGENASE FROM THE ARCHAEON SULFOLOBUS SOLFATARICUS-MONOCLINIC CRYSTAL FORM


Overview

Alcohol dehydrogenase from Sulfolobus solfataricus (SsADH) is the only enzyme from Archaea among the structurally studied members of the medium-chain ADH family described so far. Here, we present the three-dimensional structure of the apo form of the mutant N249Y which exhibits increased catalytic activity when compared to the wild-type enzyme. The substitution, located in the coenzyme binding domain, decreases the affinity for NAD(H) cofactor. The rearrangement of segments 248-250 and 270-275, induced by the mutation, suggests an explanation for the lower coenzyme affinity. This study also highlights the role in SsADH catalysis of the flexible loops located at the interface between the catalytic and the coenzyme domains.

About this Structure

1NTO is a Single protein structure of sequence from Sulfolobus solfataricus. Full crystallographic information is available from OCA.

Reference

Structural study of a single-point mutant of Sulfolobus solfataricus alcohol dehydrogenase with enhanced activity., Esposito L, Bruno I, Sica F, Raia CA, Giordano A, Rossi M, Mazzarella L, Zagari A, FEBS Lett. 2003 Mar 27;539(1-3):14-8. PMID:12650918 Page seeded by OCA on Sat May 3 02:58:01 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1nto"
Personal tools