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| | <StructureSection load='5lxy' size='340' side='right'caption='[[5lxy]], [[Resolution|resolution]] 2.85Å' scene=''> | | <StructureSection load='5lxy' size='340' side='right'caption='[[5lxy]], [[Resolution|resolution]] 2.85Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5lxy]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LXY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5LXY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5lxy]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LXY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LXY FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RBM7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), ZCCHC8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5lxy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lxy OCA], [http://pdbe.org/5lxy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lxy RCSB], [http://www.ebi.ac.uk/pdbsum/5lxy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lxy ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lxy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lxy OCA], [https://pdbe.org/5lxy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lxy RCSB], [https://www.ebi.ac.uk/pdbsum/5lxy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lxy ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/RBM7_HUMAN RBM7_HUMAN]] Possible involved in germ cell RNA processing and meiosis. [[http://www.uniprot.org/uniprot/ZCHC8_HUMAN ZCHC8_HUMAN]] May be involved in pre-mRNA splicing. | + | [https://www.uniprot.org/uniprot/RBM7_HUMAN RBM7_HUMAN] Possible involved in germ cell RNA processing and meiosis. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Benda, C]] | + | [[Category: Benda C]] |
| - | [[Category: Conti, E]] | + | [[Category: Conti E]] |
| - | [[Category: Falk, S]] | + | [[Category: Falk S]] |
| - | [[Category: Finogenova, K]] | + | [[Category: Finogenova K]] |
| - | [[Category: Next complex rrm rbm7 zcchc8]]
| + | |
| - | [[Category: Rna binding protein]]
| + | |
| Structural highlights
Function
RBM7_HUMAN Possible involved in germ cell RNA processing and meiosis.
Publication Abstract from PubMed
The eukaryotic RNA exosome participates extensively in RNA processing and degradation. In human cells, three accessory factors (RBM7, ZCCHC8 and hMTR4) interact to form the nuclear exosome targeting (NEXT) complex, which directs a subset of non-coding RNAs for exosomal degradation. Here we elucidate how RBM7 is incorporated in the NEXT complex. We identify a proline-rich segment of ZCCHC8 as the interaction site for the RNA-recognition motif (RRM) of RBM7 and present the crystal structure of the corresponding complex at 2.0 A resolution. On the basis of the structure, we identify a proline-rich segment within the splicing factor SAP145 with strong similarity to ZCCHC8. We show that this segment of SAP145 not only binds the RRM region of another splicing factor SAP49 but also the RRM of RBM7. These dual interactions of RBM7 with the exosome and the spliceosome suggest a model whereby NEXT might recruit the exosome to degrade intronic RNAs.
Structure of the RBM7-ZCCHC8 core of the NEXT complex reveals connections to splicing factors.,Falk S, Finogenova K, Melko M, Benda C, Lykke-Andersen S, Jensen TH, Conti E Nat Commun. 2016 Dec 1;7:13573. doi: 10.1038/ncomms13573. PMID:27905398[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Falk S, Finogenova K, Melko M, Benda C, Lykke-Andersen S, Jensen TH, Conti E. Structure of the RBM7-ZCCHC8 core of the NEXT complex reveals connections to splicing factors. Nat Commun. 2016 Dec 1;7:13573. doi: 10.1038/ncomms13573. PMID:27905398 doi:http://dx.doi.org/10.1038/ncomms13573
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