8j5z
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | The | + | ==The cryo-EM structure of the TwOSC1 tetramer== |
| + | <StructureSection load='8j5z' size='340' side='right'caption='[[8j5z]], [[Resolution|resolution]] 4.75Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8j5z]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Tripterygium_wilfordii Tripterygium wilfordii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8J5Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8J5Z FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.75Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8j5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8j5z OCA], [https://pdbe.org/8j5z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8j5z RCSB], [https://www.ebi.ac.uk/pdbsum/8j5z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8j5z ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A499QXI7_TRIWF A0A499QXI7_TRIWF] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The oxidosqualene cyclase (OSC) catalyzed cyclization of the linear substrate (3S)-2,3-oxidosqualene to form diverse pentacyclic triterpenoid (PT) skeletons is one of the most complex reactions in nature. Friedelin has a unique PT skeleton involving a fascinating nine-step cation shuttle run (CSR) cascade rearrangement reaction, in which the carbocation formed at C2 moves to the other side of the skeleton, runs back to C3 to yield a friedelin cation, which is finally deprotonated. However, as crystal structure data of plant OSCs are lacking, it remains unknown why the CSR cascade reactions occur in friedelin biosynthesis, as does the exact catalytic mechanism of the CSR. In this study, we determined the first cryogenic electron microscopy structure of a plant OSC, friedelin synthase, from Tripterygium wilfordii Hook. f (TwOSC). We also performed quantum mechanics/molecular mechanics simulations to reveal the energy profile for the CSR cascade reaction and identify key residues crucial for PT skeleton formation. Furthermore, we semirationally designed two TwOSC mutants, which significantly improved the yields of friedelin and beta-amyrin, respectively. | ||
| - | + | Structural and Catalytic Insight into the Unique Pentacyclic Triterpene Synthase TwOSC.,Luo Y, Ma X, Qiu Y, Lu Y, Shen S, Li Y, Gao H, Chen K, Zhou J, Hu T, Tu L, Zhao H, Li D, Leng F, Gao W, Jiang T, Liu C, Huang L, Wu R, Tong Y Angew Chem Int Ed Engl. 2023 Oct 15:e202313429. doi: 10.1002/anie.202313429. PMID:37840440<ref>PMID:37840440</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8j5z" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Tripterygium wilfordii]] | ||
| + | [[Category: Jiang T]] | ||
| + | [[Category: Ma X]] | ||
| + | [[Category: Yunfeng L]] | ||
| + | [[Category: Yuru T]] | ||
Current revision
The cryo-EM structure of the TwOSC1 tetramer
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