1oqj
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /> <applet load="1oqj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1oqj, resolution 1.55Å" /> '''Crystal structure o...)
Next diff →
Revision as of 16:29, 12 November 2007
|
Crystal structure of the SAND domain from glucocorticoid modulatory element binding protein-1 (GMEB1)
Overview
The glucocorticoid-modulatory element-binding proteins, GMEB1 and GMEB2, are ubiquitous, multifunctional DNA-binding proteins with important roles, in the modulation of transcription upon steroid hormone activation. The, GMEB proteins have intrinsic transactivation ability, but also control the, glucocorticoid response via direct binding to the glucocorticoid receptor., They are also mandatory host proteins for Parvovirus replication. Here we, present the 1.55 A resolution crystal structure of a central portion of, GMEB1, encompassing its SAND domain, which shares 80% sequence identity, with the GMEB2 SAND domain. We demonstrate that this domain, also present, in numerous proteins implicated in chromatin-associated transcriptional, regulation, is necessary and sufficient to bind the, glucocorticoid-modulatory element (GME) DNA target. We use nuclear, magnetic resonance (NMR) and binding studies to map the DNA recognition, surface to an alpha-helical region exposing the conserved KDWK motif., Using site-directed mutagenesis, key residues for DNA binding are, identified. In contrast to the previously determined NMR structure of the, Sp100b SAND domain, we find that the GMEB1 SAND domain also comprises a, zinc-binding motif. Although the zinc ion is not necessary for DNA, binding, it is found to determine the C-terminal conformation of the GMEB1, SAND domain. We also show that homologous zinc-binding motifs exist in a, subset of SAND domain proteins and probe the roles of this novel motif.
About this Structure
1OQJ is a Single protein structure of sequence from Homo sapiens with ZN as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure and nuclear magnetic resonance analyses of the SAND domain from glucocorticoid modulatory element binding protein-1 reveals deoxyribonucleic acid and zinc binding regions., Surdo PL, Bottomley MJ, Sattler M, Scheffzek K, Mol Endocrinol. 2003 Jul;17(7):1283-95. Epub 2003 Apr 17. PMID:12702733
Page seeded by OCA on Mon Nov 12 18:35:34 2007
