2zzx

From Proteopedia

(Difference between revisions)

Current revision

Crystal Structure of a Periplasmic Substrate Binding Protein in Complex with Lactate

Structural highlights

2zzx is a 4 chain structure with sequence from Thermus thermophilus HB8. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.75Å
Ligands:	, , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TLBP_THET8 Part of the tripartite ATP-independent periplasmic (TRAP) transport system involved in the uptake of lactate. This protein specifically binds L-lactate.[UniProtKB:P37676]^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Lactate is utilized in many biological processes, and its transport across biological membranes is mediated with various types of transporters. Here, we report the crystal structures of a lactate-binding protein of a TRAP (tripartite ATP-independent periplasmic) secondary transporter from Thermus thermophilus HB8. The folding of the protein is typical for a type II periplasmic solute-binding protein and forms a dimer in a back-to-back manner. One molecule of l-lactate is clearly identified in a cleft of the protein as a complex with a calcium ion. Detailed crystallographic and biochemical analyses revealed that the calcium ion can be removed from the protein and replaced with other divalent cations. This characterization of the structure of a protein binding with calcium lactate makes a significant contribution to our understanding of the mechanisms by which calcium and lactate are accommodated in cells.

Crystal structure of a periplasmic substrate-binding protein in complex with calcium lactate.,Akiyama N, Takeda K, Miki K J Mol Biol. 2009 Sep 25;392(3):559-65. Epub 2009 Jul 22. PMID:19631222^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Akiyama N, Takeda K, Miki K. Crystal structure of a periplasmic substrate-binding protein in complex with calcium lactate. J Mol Biol. 2009 Sep 25;392(3):559-65. Epub 2009 Jul 22. PMID:19631222 doi:10.1016/j.jmb.2009.07.043
↑ Akiyama N, Takeda K, Miki K. Crystal structure of a periplasmic substrate-binding protein in complex with calcium lactate. J Mol Biol. 2009 Sep 25;392(3):559-65. Epub 2009 Jul 22. PMID:19631222 doi:10.1016/j.jmb.2009.07.043

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2zzx"

Categories: Large Structures | Thermus thermophilus HB8 | Akiyama N | Miki K | Takeda K

@@ Line 3: / Line 3: @@
 <StructureSection load='2zzx' size='340' side='right'caption='[[2zzx]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2zzx]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZZX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZZX FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2zzx]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZZX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZZX FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LAC:LACTIC+ACID'>LAC</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TTHA0766 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LAC:LACTIC+ACID'>LAC</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zzx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zzx OCA], [https://pdbe.org/2zzx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zzx RCSB], [https://www.ebi.ac.uk/pdbsum/2zzx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zzx ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/TLBP_THET8 TLBP_THET8] Part of the tripartite ATP-independent periplasmic (TRAP) transport system involved in the uptake of lactate. This protein specifically binds L-lactate.[UniProtKB:P37676]<ref>PMID:19631222</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 35: / Line 37: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Thet8]]
+[[Category: Thermus thermophilus HB8]]
-[[Category: Akiyama, N]]
+[[Category: Akiyama N]]
-[[Category: Miki, K]]
+[[Category: Miki K]]
-[[Category: Takeda, K]]
+[[Category: Takeda K]]
-[[Category: Lactate]]
-[[Category: Periplasmic substrate binding protein]]
-[[Category: Transport protein]]
-[[Category: Trap transporter]]

2zzx

From Proteopedia

Current revision

Crystal Structure of a Periplasmic Substrate Binding Protein in Complex with Lactate

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox