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| | <StructureSection load='3b6d' size='340' side='right'caption='[[3b6d]], [[Resolution|resolution]] 1.20Å' scene=''> | | <StructureSection load='3b6d' size='340' side='right'caption='[[3b6d]], [[Resolution|resolution]] 1.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3b6d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Strs0 Strs0]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B6D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B6D FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3b6d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._SA-COO Streptomyces sp. SA-COO]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B6D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B6D FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAE:FLAVIN-N7+PROTONATED-ADENINE+DINUCLEOTIDE'>FAE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3b3r|3b3r]]</div></td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAE:FLAVIN-N7+PROTONATED-ADENINE+DINUCLEOTIDE'>FAE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">choA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=74576 STRS0])</td></tr> | + | |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Cholesterol_oxidase Cholesterol oxidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.6 1.1.3.6] </span></td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b6d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b6d OCA], [https://pdbe.org/3b6d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b6d RCSB], [https://www.ebi.ac.uk/pdbsum/3b6d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b6d ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b6d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b6d OCA], [https://pdbe.org/3b6d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b6d RCSB], [https://www.ebi.ac.uk/pdbsum/3b6d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b6d ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/CHOD_STRS0 CHOD_STRS0]] Bifunctional enzyme that catalyzes the oxidation of the 3-beta-hydroxy group of cholesterol and the isomerization of the double bond of the resulting product.
| + | [https://www.uniprot.org/uniprot/CHOD_STRS0 CHOD_STRS0] Bifunctional enzyme that catalyzes the oxidation of the 3-beta-hydroxy group of cholesterol and the isomerization of the double bond of the resulting product. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Cholesterol oxidase]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Strs0]] | + | [[Category: Streptomyces sp. SA-COO]] |
| - | [[Category: Lyubimov, A Y]] | + | [[Category: Lyubimov AY]] |
| - | [[Category: Vrielink, A]] | + | [[Category: Vrielink A]] |
| - | [[Category: Cholesterol metabolism]]
| + | |
| - | [[Category: Fad]]
| + | |
| - | [[Category: Flavin]]
| + | |
| - | [[Category: Flavin activation]]
| + | |
| - | [[Category: Flavoenzyme]]
| + | |
| - | [[Category: Flavoprotein]]
| + | |
| - | [[Category: Lipid metabolism]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| - | [[Category: Secreted]]
| + | |
| - | [[Category: Steroid metabolism]]
| + | |
| Structural highlights
Function
CHOD_STRS0 Bifunctional enzyme that catalyzes the oxidation of the 3-beta-hydroxy group of cholesterol and the isomerization of the double bond of the resulting product.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Two high-resolution structures of a double mutant of bacterial cholesterol oxidase in the presence or absence of a ligand, glycerol, are presented, showing the trajectory of glycerol as it binds in a Michaelis complex-like position in the active site. A group of three aromatic residues forces the oxidized isoalloxazine moiety to bend along the N5-N10 axis as a response to the binding of glycerol in the active site. Movement of these aromatic residues is only observed in the glycerol-bound structure, indicating that some tuning of the FAD redox potential is caused by the formation of the Michaelis complex during regular catalysis. This structural study suggests a possible mechanism of substrate-assisted flavin activation, improves our understanding of the interplay between the enzyme, its flavin cofactor and its substrate, and is of use to the future design of effective cholesterol oxidase inhibitors.
Distortion of flavin geometry is linked to ligand binding in cholesterol oxidase.,Lyubimov AY, Heard K, Tang H, Sampson NS, Vrielink A Protein Sci. 2007 Dec;16(12):2647-56. PMID:18029419[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Lyubimov AY, Heard K, Tang H, Sampson NS, Vrielink A. Distortion of flavin geometry is linked to ligand binding in cholesterol oxidase. Protein Sci. 2007 Dec;16(12):2647-56. PMID:18029419 doi:16/12/2647
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