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| | <StructureSection load='3bsb' size='340' side='right'caption='[[3bsb]], [[Resolution|resolution]] 2.80Å' scene=''> | | <StructureSection load='3bsb' size='340' side='right'caption='[[3bsb]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3bsb]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BSB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BSB FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3bsb]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BSB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BSB FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1m8y|1m8y]], [[1m8x|1m8x]], [[1m8w|1m8w]], [[3bsx|3bsx]]</div></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PUM1, KIAA0099, PUMH1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bsb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bsb OCA], [https://pdbe.org/3bsb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bsb RCSB], [https://www.ebi.ac.uk/pdbsum/3bsb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bsb ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bsb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bsb OCA], [https://pdbe.org/3bsb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bsb RCSB], [https://www.ebi.ac.uk/pdbsum/3bsb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bsb ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/PUM1_HUMAN PUM1_HUMAN]] Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. May be required to support proliferation and self-renewal of stem cells (By similarity).
| + | [https://www.uniprot.org/uniprot/PUM1_HUMAN PUM1_HUMAN] Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. May be required to support proliferation and self-renewal of stem cells (By similarity). |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Aggarwal, A K]] | + | [[Category: Aggarwal AK]] |
| - | [[Category: Gupta, Y K]] | + | [[Category: Gupta YK]] |
| - | [[Category: Nair, D T]] | + | [[Category: Nair DT]] |
| - | [[Category: Wharton, R P]] | + | [[Category: Wharton RP]] |
| - | [[Category: Alternative splicing]]
| + | |
| - | [[Category: Cytoplasm]]
| + | |
| - | [[Category: Phosphoprotein]]
| + | |
| - | [[Category: Protein-rna complex]]
| + | |
| - | [[Category: Rna binding protein-rna complex]]
| + | |
| - | [[Category: Rna-binding]]
| + | |
| - | [[Category: Translation regulation]]
| + | |
| Structural highlights
Function
PUM1_HUMAN Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. May be required to support proliferation and self-renewal of stem cells (By similarity).
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Pumilio is a founder member of the evolutionarily conserved Puf family of RNA-binding proteins that control a number of physiological processes in eukaryotes. A structure of human Pumilio (hPum) Puf domain bound to a Drosophila regulatory sequence showed that each Puf repeat recognizes a single nucleotide. Puf domains in general bind promiscuously to a large set of degenerate sequences, but the structural basis for this promiscuity has been unclear. Here, we describe the structures of hPum Puf domain complexed to two noncognate RNAs, CycB(reverse) and Puf5. In each complex, one of the nucleotides is ejected from the binding surface, in effect, acting as a "spacer." The complexes also reveal the plasticity of several Puf repeats, which recognize noncanonical nucleotides. Together, these complexes provide a molecular basis for recognition of degenerate binding sites, which significantly increases the number of mRNAs targeted for regulation by Puf proteins in vivo.
Structures of human Pumilio with noncognate RNAs reveal molecular mechanisms for binding promiscuity.,Gupta YK, Nair DT, Wharton RP, Aggarwal AK Structure. 2008 Apr;16(4):549-57. Epub 2008 Mar 6. PMID:18328718[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gupta YK, Nair DT, Wharton RP, Aggarwal AK. Structures of human Pumilio with noncognate RNAs reveal molecular mechanisms for binding promiscuity. Structure. 2008 Apr;16(4):549-57. Epub 2008 Mar 6. PMID:18328718 doi:10.1016/j.str.2008.01.006
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