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1ou5
From Proteopedia
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(New page: 200px<br /> <applet load="1ou5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ou5, resolution 3.40Å" /> '''Crystal structure o...)
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Revision as of 16:29, 12 November 2007
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Crystal structure of human CCA-adding enzyme
Contents |
Overview
All tRNA molecules carry the invariant sequence CCA at their 3'-terminus, for amino acid attachment. The post-transcriptional addition of CCA is, carried out by ATP(CTP):tRNA nucleotidyltransferase, also called CCase., This enzyme catalyses a unique template-independent but sequence-specific, nucleotide polymerization reaction. In order to reveal the molecular, mechanism of this activity, we solved the crystal structure of human CCase, by single isomorphous replacement. The structure reveals a four domain, architecture with a cluster of conserved residues forming a positively, charged cleft between the first two domains. Structural homology of the, N-terminal CCase domain to other nucleotidyltransferases could be, exploited for modeling a tRNA-substrate complex. The model places the tRNA, 3'-end into the N-terminal nucleotidyltransferase site, close to a patch, of conserved residues that provide the binding sites for CTP and ATP., Based on our results, we introduce a corkscrew model for CCA addition that, includes a fixed active site and a traveling tRNA-binding region formed by, flexible parts of the protein.
Disease
Known disease associated with this structure: Deafness, mitochondrial, modifier of OMIM:[610230]
About this Structure
1OU5 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of the human CCA-adding enzyme: insights into template-independent polymerization., Augustin MA, Reichert AS, Betat H, Huber R, Morl M, Steegborn C, J Mol Biol. 2003 May 16;328(5):985-94. PMID:12729736
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