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| | <StructureSection load='3fk6' size='340' side='right'caption='[[3fk6]], [[Resolution|resolution]] 2.10Å' scene=''> | | <StructureSection load='3fk6' size='340' side='right'caption='[[3fk6]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3fk6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FK6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FK6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3fk6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FK6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FK6 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2vke|2vke]], [[2o7o|2o7o]], [[2vkv|2vkv]], [[2tct|2tct]], [[3fk7|3fk7]]</div></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tetR ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
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| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fk6 OCA], [https://pdbe.org/3fk6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fk6 RCSB], [https://www.ebi.ac.uk/pdbsum/3fk6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fk6 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fk6 OCA], [https://pdbe.org/3fk6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fk6 RCSB], [https://www.ebi.ac.uk/pdbsum/3fk6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fk6 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/TETR2_ECOLX TETR2_ECOLX]] TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.
| + | [https://www.uniprot.org/uniprot/TETR4_ECOLX TETR4_ECOLX] TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus coli migula 1895]] | + | [[Category: Escherichia coli]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Gmeiner, P]] | + | [[Category: Gmeiner P]] |
| - | [[Category: Hillen, W]] | + | [[Category: Hillen W]] |
| - | [[Category: Klieber, M A]] | + | [[Category: Klieber MA]] |
| - | [[Category: Lochner, S]] | + | [[Category: Lochner S]] |
| - | [[Category: Muller, Y A]] | + | [[Category: Muller YA]] |
| - | [[Category: Scholz, O]] | + | [[Category: Scholz O]] |
| - | [[Category: 4-de-dimethylamino-anhydrotetracycline]]
| + | |
| - | [[Category: Altered inducer specificity]]
| + | |
| - | [[Category: Antibiotic resistance]]
| + | |
| - | [[Category: Bacterial transcription regulation]]
| + | |
| - | [[Category: Dna-binding]]
| + | |
| - | [[Category: Magnesium]]
| + | |
| - | [[Category: Metal-binding]]
| + | |
| - | [[Category: Repressor]]
| + | |
| - | [[Category: Tetracycline repressor]]
| + | |
| - | [[Category: Transcription]]
| + | |
| - | [[Category: Transcription regulation]]
| + | |
| - | [[Category: Transposable element]]
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| Structural highlights
Function
TETR4_ECOLX TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The bacterial tetracycline transcription regulation system mediated by the tetracycline repressor (TetR) is widely used to study gene expression in prokaryotes and eukaryotes. To study multiple genes in parallel, a triple mutant TetR(K(64)L(135)I(138)) has been engineered that is selectively induced by the synthetic tetracycline derivative 4-de-dimethylamino-anhydrotetracycline (4-ddma-atc) and no longer by tetracycline, the inducer of wild-type TetR. In the present study, we report the crystal structure of TetR(K(64)L(135)I(138)) in the absence and in complex with 4-ddma-atc at resolutions of 2.1 A. Analysis of the structures in light of the available binding data and previously reported TetR complexes allows for a dissection of the origins of selectivity and specificity. In all crystal structures solved to date, the ligand-binding position, as well as the positioning of the residues lining the binding site, is extremely well conserved, irrespective of the chemical nature of the ligand. Selective recognition of 4-ddma-atc is achieved through fine-tuned hydrogen-bonding constraints introduced by the His64-->Lys substitution, as well as a combination of hydrophobic effect and the removal of unfavorable electrostatic interactions through the introduction of Leu135 and Ile138.
Structural origins for selectivity and specificity in an engineered bacterial repressor-inducer pair.,Klieber MA, Scholz O, Lochner S, Gmeiner P, Hillen W, Muller YA FEBS J. 2009 Oct;276(19):5610-21. Epub 2009 Aug 27. PMID:19712110[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Klieber MA, Scholz O, Lochner S, Gmeiner P, Hillen W, Muller YA. Structural origins for selectivity and specificity in an engineered bacterial repressor-inducer pair. FEBS J. 2009 Oct;276(19):5610-21. Epub 2009 Aug 27. PMID:19712110 doi:10.1111/j.1742-4658.2009.07254.x
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