3gfl

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of the ST1710 mutant (R90A) protein

Structural highlights

3gfl is a 1 chain structure with sequence from Sulfurisphaera tokodaii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.9Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Function

Q96ZY1_SULTO

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

ST1710, a member of the multiple antibiotic resistance regulator (MarR) family of regulatory proteins in bacteria and archaea, plays important roles in development of antibiotic resistance, a global health problem. Here, we present the crystal structure of ST1710 from Sulfolobus tokodaii strain 7 complexed with salicylate, a well-known inhibitor of MarR proteins and the ST1710 complex with its promoter DNA, refined to 1.8 and 2.10 A resolutions, respectively. The ST1710-DNA complex shares the topology of apo-ST1710 and MarR proteins, with each subunit containing a winged helix-turn-helix (wHtH) DNA binding motif. Significantly large conformational changes occurred upon DNA binding and in each of the dimeric monomers in the asymmetric unit of the ST1710-DNA complex. Conserved wHtH loop residues interacting with the bound DNA and mutagenic analysis indicated that R89, R90 and K91 were important for DNA recognition. Significantly, the bound DNA exhibited a new binding mechanism.

ST1710-DNA complex crystal structure reveals the DNA binding mechanism of the MarR family of regulators.,Kumarevel T, Tanaka T, Umehara T, Yokoyama S Nucleic Acids Res. 2009 Aug;37(14):4723-35. Epub 2009 Jun 9. PMID:19509310^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kumarevel T, Tanaka T, Umehara T, Yokoyama S. ST1710-DNA complex crystal structure reveals the DNA binding mechanism of the MarR family of regulators. Nucleic Acids Res. 2009 Aug;37(14):4723-35. Epub 2009 Jun 9. PMID:19509310 doi:10.1093/nar/gkp496

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3gfl"

Categories: Large Structures | Sulfurisphaera tokodaii | Kumarevel T | Tanaka T | Yokoyama S

@@ Line 3: / Line 3: @@
 <StructureSection load='3gfl' size='340' side='right'caption='[[3gfl]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3gfl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dsm_16993 Dsm 16993]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GFL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GFL FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3gfl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfurisphaera_tokodaii Sulfurisphaera tokodaii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GFL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GFL FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2eb7|2eb7]], [[2yr2|2yr2]], [[3gez|3gez]], [[3gf2|3gf2]], [[3gfi|3gfi]], [[3gfj|3gfj]], [[3gfm|3gfm]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ST1710 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=111955 DSM 16993])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gfl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gfl OCA], [https://pdbe.org/3gfl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gfl RCSB], [https://www.ebi.ac.uk/pdbsum/3gfl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gfl ProSAT], [https://www.topsan.org/Proteins/RSGI/3gfl TOPSAN]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q96ZY1_SULTO Q96ZY1_SULTO]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 32: / Line 33: @@
 __TOC__
 </StructureSection>
-[[Category: Dsm 16993]]
 [[Category: Large Structures]]
-[[Category: Kumarevel, T]]
+[[Category: Sulfurisphaera tokodaii]]
-[[Category: Structural genomic]]
+[[Category: Kumarevel T]]
-[[Category: Tanaka, T]]
+[[Category: Tanaka T]]
-[[Category: Yokoyama, S]]
+[[Category: Yokoyama S]]
-[[Category: Marr]]
-[[Category: National project on protein structural and functional analyse]]
-[[Category: Nppsfa]]
-[[Category: Rsgi]]
-[[Category: St1710]]
-[[Category: Transcription]]
-[[Category: Transcription regulation]]
-[[Category: Transcription regulator]]

3gfl

From Proteopedia

Current revision

Crystal structure of the ST1710 mutant (R90A) protein

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox