3gzj

From Proteopedia

(Difference between revisions)

Current revision

Crystal Structure of Polyneuridine Aldehyde Esterase Complexed with 16-epi-Vellosimine

Structural highlights

3gzj is a 5 chain structure with sequence from Rauvolfia serpentina. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.19Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PNAE_RAUSE Catalyzes the hydrolysis of polyneuridine aldehyde into epi-vellosimine, which is the immediate precursor for the synthesis of the ajmaline.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Cutting carbons: The three-dimensional structure of polyneuridine aldehyde esterase (PNAE) gives insight into the enzymatic mechanism of the biosynthesis of C(9)- from C(10)-monoterpenoid indole alkaloids (see scheme). PNAE is a very substrate-specific serine esterase. It harbors the catalytic triad S87-D216-H244, and is a new member of the alpha/beta-fold hydrolase superfamily. Its novel function leads to the diversification of alkaloid structures.

Structural basis and enzymatic mechanism of the biosynthesis of C9- from C10-monoterpenoid indole alkaloids.,Yang L, Hill M, Wang M, Panjikar S, Stockigt J Angew Chem Int Ed Engl. 2009;48(28):5211-3. PMID:19496101^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Dogru E, Warzecha H, Seibel F, Haebel S, Lottspeich F, Stockigt J. The gene encoding polyneuridine aldehyde esterase of monoterpenoid indole alkaloid biosynthesis in plants is an ortholog of the alpha/betahydrolase super family. Eur J Biochem. 2000 Mar;267(5):1397-406. PMID:10691977
↑ Yang L, Hill M, Wang M, Panjikar S, Stockigt J. Structural basis and enzymatic mechanism of the biosynthesis of C9- from C10-monoterpenoid indole alkaloids. Angew Chem Int Ed Engl. 2009;48(28):5211-3. PMID:19496101 doi:10.1002/anie.200900150

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3gzj"

@@ Line 3: / Line 3: @@
 <StructureSection load='3gzj' size='340' side='right'caption='[[3gzj]], [[Resolution|resolution]] 2.19&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3gzj]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Ophioxylon_serpentinum Ophioxylon serpentinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GZJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GZJ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3gzj]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Rauvolfia_serpentina Rauvolfia serpentina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GZJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GZJ FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EVS:16-EPI-VELLOSIMINE'>EVS</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.19&#8491;</td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Polyneuridine-aldehyde_esterase Polyneuridine-aldehyde esterase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.78 3.1.1.78] </span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EVS:16-EPI-VELLOSIMINE'>EVS</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gzj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gzj OCA], [https://pdbe.org/3gzj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gzj RCSB], [https://www.ebi.ac.uk/pdbsum/3gzj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gzj ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/PNAE_RAUSE PNAE_RAUSE]] Catalyzes the hydrolysis of polyneuridine aldehyde into epi-vellosimine, which is the immediate precursor for the synthesis of the ajmaline.<ref>PMID:10691977</ref>
+[https://www.uniprot.org/uniprot/PNAE_RAUSE PNAE_RAUSE] Catalyzes the hydrolysis of polyneuridine aldehyde into epi-vellosimine, which is the immediate precursor for the synthesis of the ajmaline.<ref>PMID:10691977</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 37: / Line 37: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Ophioxylon serpentinum]]
+[[Category: Rauvolfia serpentina]]
-[[Category: Polyneuridine-aldehyde esterase]]
+[[Category: Hill M]]
-[[Category: Hill, M]]
+[[Category: Panjikar S]]
-[[Category: Panjikar, S]]
+[[Category: Stoeckigt J]]
-[[Category: Stoeckigt, J]]
+[[Category: Wang M]]
-[[Category: Wang, M]]
+[[Category: Yang L]]
-[[Category: Yang, L]]
-[[Category: Alkaloid metabolism]]
-[[Category: Hydrolase]]
-[[Category: Hydrolase superfamily]]
-[[Category: Serine esterase]]

3gzj

From Proteopedia

Current revision

Crystal Structure of Polyneuridine Aldehyde Esterase Complexed with 16-epi-Vellosimine

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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