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| | <StructureSection load='3i1u' size='340' side='right'caption='[[3i1u]], [[Resolution|resolution]] 1.39Å' scene=''> | | <StructureSection load='3i1u' size='340' side='right'caption='[[3i1u]], [[Resolution|resolution]] 1.39Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3i1u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I1U OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3I1U FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3i1u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I1U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I1U FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTW:(2S,3R)-2-BENZYL-3-SULFANYLBUTANOIC+ACID'>BTW</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.391Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxypeptidase_A Carboxypeptidase A], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.1 3.4.17.1] </span></td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTW:(2S,3R)-2-BENZYL-3-SULFANYLBUTANOIC+ACID'>BTW</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3i1u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i1u OCA], [http://pdbe.org/3i1u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3i1u RCSB], [http://www.ebi.ac.uk/pdbsum/3i1u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3i1u ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i1u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i1u OCA], [https://pdbe.org/3i1u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i1u RCSB], [https://www.ebi.ac.uk/pdbsum/3i1u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i1u ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/CBPA1_BOVIN CBPA1_BOVIN] Carboxypeptidase that catalyzes the release of a C-terminal amino acid, but has little or no action with -Asp, -Glu, -Arg, -Lys or -Pro (By similarity). |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Bos taurus]] | | [[Category: Bos taurus]] |
| - | [[Category: Carboxypeptidase A]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Fernandez, D]] | + | [[Category: Fernandez D]] |
| - | [[Category: Carboxypeptidase some]]
| + | |
| - | [[Category: Covalently-modified protein]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Thiirane]]
| + | |
| - | [[Category: Zinc-dependent protease]]
| + | |
| Structural highlights
Function
CBPA1_BOVIN Carboxypeptidase that catalyzes the release of a C-terminal amino acid, but has little or no action with -Asp, -Glu, -Arg, -Lys or -Pro (By similarity).
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The three-dimensional X-ray crystal structure of carboxypeptidase A, a zinc-dependent hydrolase, covalently modified by a mechanism-based thiirane inactivator, 2-benzyl-3,4-epithiobutanoic acid, has been solved to 1.38 A resolution. The interaction of the thiirane moiety of the inhibitor with the active site zinc ion promotes its covalent modification of Glu-270 with the attendant opening of the thiirane ring. The crystal structure determination at high resolution allowed for the clear visualization of the covalent ester bond to the glutamate side chain. The newly generated thiol from the inhibitor binds to the catalytic zinc ion in a monodentate manner, inducing a change in the zinc ion geometry and coordination, while its benzyl group fits into the S1' specificity pocket of the enzyme. The inhibitor molecule is distorted at the position of the carbon atom that is involved in the ester bond linkage on one side and the zinc coordination on the other. This particular type of thiirane-based metalloprotease inhibitor is for the first time analyzed in complex to the target protease at high resolution and may be used as a general model for zinc-dependent proteases.
The X-ray structure of carboxypeptidase A inhibited by a thiirane mechanism-based inhibitor.,Fernandez D, Testero S, Vendrell J, Aviles FX, Mobashery S Chem Biol Drug Des. 2010 Jan;75(1):29-34. Epub 2009 Nov 4. PMID:19895506[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Fernandez D, Testero S, Vendrell J, Aviles FX, Mobashery S. The X-ray structure of carboxypeptidase A inhibited by a thiirane mechanism-based inhibitor. Chem Biol Drug Des. 2010 Jan;75(1):29-34. Epub 2009 Nov 4. PMID:19895506 doi:10.1111/j.1747-0285.2009.00907.x
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