|
|
| Line 3: |
Line 3: |
| | <StructureSection load='3ipv' size='340' side='right'caption='[[3ipv]], [[Resolution|resolution]] 2.04Å' scene=''> | | <StructureSection load='3ipv' size='340' side='right'caption='[[3ipv]], [[Resolution|resolution]] 2.04Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3ipv]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Spatholobus_parviflorus Spatholobus parviflorus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IPV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3IPV FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3ipv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Spatholobus_parviflorus Spatholobus parviflorus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IPV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IPV FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.04Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3ipv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ipv OCA], [http://pdbe.org/3ipv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ipv RCSB], [http://www.ebi.ac.uk/pdbsum/3ipv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ipv ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ipv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ipv OCA], [https://pdbe.org/3ipv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ipv RCSB], [https://www.ebi.ac.uk/pdbsum/3ipv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ipv ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/LECA_SPAPA LECA_SPAPA] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| Line 33: |
Line 35: |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| | [[Category: Spatholobus parviflorus]] | | [[Category: Spatholobus parviflorus]] |
| - | [[Category: Abhilash, J]] | + | [[Category: Abhilash J]] |
| - | [[Category: Bharath, S R]] | + | [[Category: Bharath SR]] |
| - | [[Category: Geethanandan, K]] | + | [[Category: Geethanandan K]] |
| - | [[Category: Haridas, M]] | + | [[Category: Haridas M]] |
| - | [[Category: Sadasivan, C]] | + | [[Category: Sadasivan C]] |
| - | [[Category: Anti fungal]]
| + | |
| - | [[Category: Galactose binding]]
| + | |
| - | [[Category: Hemagglutinin]]
| + | |
| - | [[Category: Legume lectin]]
| + | |
| - | [[Category: Seed lectin]]
| + | |
| - | [[Category: Sugar binding protein]]
| + | |
| Structural highlights
Function
LECA_SPAPA
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
A galactose-specific seed lectin from Spatholobous parviflorus (SPL) has been purified, crystallized and its X-ray structure solved. It is the first lectin purified and crystallized from the genus Spatholobus (family: Fabaceae). The crystals belong to the space group P1, with a=60.792 A, b=60.998 A, c=78.179 A, alpha=78.68 degrees , beta=88.62 degrees , gamma=104.32 degrees . The data were collected at 2.04 A resolution under cryocondition, on a MAR image-plate detector system, mounted on a rotating anode X-ray generator. The coordinates of Dolichos biflorus lectin (1lu1) were successfully used for the structure solution by molecular replacement method. The primary structure of the SPL was not known earlier and it was unambiguously visible in the electron density. S. parviflorus lectin is a hetero-dimeric-tetramer with two alpha and two beta chains of 251 and 239 residues respectively. SPL has two metal ions, Ca(2+) and Mn(2+), bound to a loop region of each chain. The SPL monomers are in jelly roll form.
X-ray structure of a galactose-specific lectin from Spatholobous parviflorous.,Geethanandan K, Abhilash J, Bharath SR, Sadasivan C, Haridas M Int J Biol Macromol. 2011 Dec 1;49(5):992-8. Epub 2011 Aug 26. PMID:21889532[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Geethanandan K, Abhilash J, Bharath SR, Sadasivan C, Haridas M. X-ray structure of a galactose-specific lectin from Spatholobous parviflorous. Int J Biol Macromol. 2011 Dec 1;49(5):992-8. Epub 2011 Aug 26. PMID:21889532 doi:10.1016/j.ijbiomac.2011.08.021
|
