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| | <StructureSection load='3ir1' size='340' side='right'caption='[[3ir1]], [[Resolution|resolution]] 2.15Å' scene=''> | | <StructureSection load='3ir1' size='340' side='right'caption='[[3ir1]], [[Resolution|resolution]] 2.15Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3ir1]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/"diplokokkus_intracellularis_meningitidis"_(sic)_weichselbaum_1887 "diplokokkus intracellularis meningitidis" (sic) weichselbaum 1887]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IR1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IR1 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3ir1]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_meningitidis Neisseria meningitidis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IR1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IR1 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MET:METHIONINE'>MET</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3gxa|3gxa]]</div></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MET:METHIONINE'>MET</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gna1946 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=487 "Diplokokkus intracellularis meningitidis" (sic) Weichselbaum 1887])</td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ir1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ir1 OCA], [https://pdbe.org/3ir1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ir1 RCSB], [https://www.ebi.ac.uk/pdbsum/3ir1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ir1 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ir1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ir1 OCA], [https://pdbe.org/3ir1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ir1 RCSB], [https://www.ebi.ac.uk/pdbsum/3ir1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ir1 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q7BMQ8_NEIME Q7BMQ8_NEIME] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Shen, Y]] | + | [[Category: Neisseria meningitidis]] |
| - | [[Category: Wang, X]] | + | [[Category: Shen Y]] |
| - | [[Category: Wu, Z]] | + | [[Category: Wang X]] |
| - | [[Category: Yang, X]] | + | [[Category: Wu Z]] |
| - | [[Category: D-methionine cultured]]
| + | [[Category: Yang X]] |
| - | [[Category: Gna1946]]
| + | |
| - | [[Category: Lipoprotein]]
| + | |
| - | [[Category: Protein binding]]
| + | |
| Structural highlights
Function
Q7BMQ8_NEIME
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
GNA1946, a conserved outer membrane lipoprotein from Neisseria meningitidis, has been identified as a candidate antigen for an urgently needed broad-spectrum meningococcal vaccine. It has been predicted to be a periplasmic receptor in the D-methionine uptake ABC transporter system. The crystal structure of GNA1946 was solved by the single-wavelength anomalous dispersion (SAD) method to a resolution of 2.25 A, and it reveals a Venus flytrap-like structure. GNA1946 consists of two globular lobes connected by a hinge region. Surprisingly, the structure showed an L-methionine bound within the cleft between the lobes. A comparison of GNA1946 with two other outer membrane lipoproteins, the L-methionine-binding Tp32 from Treponema pallidum and the dipeptide GlyMet-binding protein Pg110 from Staphylococcus aureus, revealed that although these three proteins share low sequence similarities, there is a high degree of structural conservation and similar substrate-binding frameworks. Our results reveal that GNA1946 is an L-methionine binding lipoprotein in the outer membrane, and should function as an initial receptor for ABC transporters with high affinity and specificity. The GNA1946 structure reported here should provide a valuable starting point for the development of a broad-spectrum meningococcal vaccine.
Crystal structure of lipoprotein GNA1946 from Neisseria meningitidis.,Yang X, Wu Z, Wang X, Yang C, Xu H, Shen Y J Struct Biol. 2009 Dec;168(3):437-43. Epub 2009 Sep 3. PMID:19733245[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Yang X, Wu Z, Wang X, Yang C, Xu H, Shen Y. Crystal structure of lipoprotein GNA1946 from Neisseria meningitidis. J Struct Biol. 2009 Dec;168(3):437-43. Epub 2009 Sep 3. PMID:19733245 doi:10.1016/j.jsb.2009.09.001
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