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| | ==Crystal structure of D138L mutant of Catabolite Gene Activator Protein== | | ==Crystal structure of D138L mutant of Catabolite Gene Activator Protein== |
| - | <StructureSection load='3kcc' size='340' side='right' caption='[[3kcc]], [[Resolution|resolution]] 1.66Å' scene=''> | + | <StructureSection load='3kcc' size='340' side='right'caption='[[3kcc]], [[Resolution|resolution]] 1.66Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3kcc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KCC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KCC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3kcc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KCC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KCC FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CMP:ADENOSINE-3,5-CYCLIC-MONOPHOSPHATE'>CMP</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.66Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">crp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CMP:ADENOSINE-3,5-CYCLIC-MONOPHOSPHATE'>CMP</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kcc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kcc OCA], [http://pdbe.org/3kcc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3kcc RCSB], [http://www.ebi.ac.uk/pdbsum/3kcc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3kcc ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3kcc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kcc OCA], [https://pdbe.org/3kcc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3kcc RCSB], [https://www.ebi.ac.uk/pdbsum/3kcc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3kcc ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CRP_ECOLI CRP_ECOLI]] This protein complexes with cyclic AMP and binds to specific DNA sites near the promoter to regulate the transcription of several catabolite-sensitive operons. The protein induces a severe bend in the DNA. Acts as a negative regulator of its own synthesis as well as for adenylate cyclase (cyaA), which generates cAMP.<ref>PMID:2982847</ref> | + | [https://www.uniprot.org/uniprot/CRP_ECOLI CRP_ECOLI] This protein complexes with cyclic AMP and binds to specific DNA sites near the promoter to regulate the transcription of several catabolite-sensitive operons. The protein induces a severe bend in the DNA. Acts as a negative regulator of its own synthesis as well as for adenylate cyclase (cyaA), which generates cAMP.<ref>PMID:2982847</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | ==See Also== | | ==See Also== |
| - | *[[Catabolite gene activator protein|Catabolite gene activator protein]] | + | *[[Catabolite gene activator protein 3D structures|Catabolite gene activator protein 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Ecoli]] | + | [[Category: Escherichia coli K-12]] |
| - | [[Category: Dong, Y H]] | + | [[Category: Large Structures]] |
| - | [[Category: Gao, Z Q]] | + | [[Category: Dong YH]] |
| - | [[Category: Tao, W B]] | + | [[Category: Gao ZQ]] |
| - | [[Category: Yu, S N]] | + | [[Category: Tao WB]] |
| - | [[Category: Zhou, J H]] | + | [[Category: Yu SN]] |
| - | [[Category: Activator]] | + | [[Category: Zhou JH]] |
| - | [[Category: Camp]]
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| - | [[Category: Camp-binding]]
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| - | [[Category: Dna-binding]]
| + | |
| - | [[Category: Helix-turn-helix]]
| + | |
| - | [[Category: Nucleotide-binding]]
| + | |
| - | [[Category: Transcription]]
| + | |
| - | [[Category: Transcription regulation]]
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| Structural highlights
Function
CRP_ECOLI This protein complexes with cyclic AMP and binds to specific DNA sites near the promoter to regulate the transcription of several catabolite-sensitive operons. The protein induces a severe bend in the DNA. Acts as a negative regulator of its own synthesis as well as for adenylate cyclase (cyaA), which generates cAMP.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The X-ray crystal structure of the cAMP-liganded D138L mutant of Escherichia coli catabolite gene activator protein (CAP) was determined at a resolution of 1.66A. This high resolution crystal structure reveals four cAMP binding sites in the homodimer. Two anti conformations of cAMPs (anti-cAMP) locate between the beta-barrel and the C-helix of each subunit; two syn conformations of cAMPs (syn-cAMP) bind on the surface of the C-terminal domain. With two syn-cAMP molecules bound, the D138L CAP is highly symmetrical with both subunits assuming a "closed" conformation. These differences make the hinge region of the mutant more flexible. Protease susceptibility measurements indicate that D138L is more susceptible to proteases than that of wild type (WT) CAP. The results of protein dynamic experiments (H/D exchange measurements) indicate that the structure of D138L mutant is more dynamic than that of WT CAP, which may impact the recognition of specific DNA sequences.
The 1.6A resolution structure of activated D138L mutant of catabolite gene activator protein with two cAMP bound in each monomer.,Tao W, Gao Z, Gao Z, Zhou J, Huang Z, Dong Y, Yu S Int J Biol Macromol. 2011 Apr 1;48(3):459-65. Epub 2011 Jan 19. PMID:21255606[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Aiba H. Transcription of the Escherichia coli adenylate cyclase gene is negatively regulated by cAMP-cAMP receptor protein. J Biol Chem. 1985 Mar 10;260(5):3063-70. PMID:2982847
- ↑ Tao W, Gao Z, Gao Z, Zhou J, Huang Z, Dong Y, Yu S. The 1.6A resolution structure of activated D138L mutant of catabolite gene activator protein with two cAMP bound in each monomer. Int J Biol Macromol. 2011 Apr 1;48(3):459-65. Epub 2011 Jan 19. PMID:21255606 doi:10.1016/j.ijbiomac.2011.01.009
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