3l4g

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of Homo Sapiens cytoplasmic Phenylalanyl-tRNA synthetase

Structural highlights

3l4g is a 16 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.3Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SYFA_HUMAN

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The existence of three types of phenylalanyl-tRNA synthetase (PheRS), bacterial (alphabeta)(2), eukaryotic/archaeal cytosolic (alphabeta)(2), and mitochondrial alpha, is a prominent example of structural diversity within the aaRS family. PheRSs have considerably diverged in primary sequences, domain compositions, and subunit organizations. Loss of the anticodon-binding domain B8 in human cytosolic PheRS (hcPheRS) is indicative of variations in the tRNA(Phe) binding and recognition as compared to bacterial PheRSs. We report herein the crystal structure of hcPheRS in complex with phenylalanine at 3.3 A resolution. A novel structural module has been revealed at the N terminus of the alpha subunit. It stretches out into the solvent of approximately 80 A and is made up of three structural domains (DBDs) possessing DNA-binding fold. The dramatic reduction of aminoacylation activity for truncated N terminus variants coupled with structural data and tRNA-docking model testify that DBDs play crucial role in hcPheRS activity.

Structure of human cytosolic phenylalanyl-tRNA synthetase: evidence for kingdom-specific design of the active sites and tRNA binding patterns.,Finarov I, Moor N, Kessler N, Klipcan L, Safro MG Structure. 2010 Mar 10;18(3):343-53. PMID:20223217^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Finarov I, Moor N, Kessler N, Klipcan L, Safro MG. Structure of human cytosolic phenylalanyl-tRNA synthetase: evidence for kingdom-specific design of the active sites and tRNA binding patterns. Structure. 2010 Mar 10;18(3):343-53. PMID:20223217 doi:10.1016/j.str.2010.01.002

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3l4g"

@@ Line 1: / Line 1: @@
 ==Crystal structure of Homo Sapiens cytoplasmic Phenylalanyl-tRNA synthetase==
-<StructureSection load='3l4g' size='340' side='right' caption='[[3l4g]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
+<StructureSection load='3l4g' size='340' side='right'caption='[[3l4g]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3l4g]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L4G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3L4G FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3l4g]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L4G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3L4G FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PHE:PHENYLALANINE'>PHE</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FARSA, FARS, FARSL, FARSLA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), FARSB, FARSLB, FRSB, HSPC173 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PHE:PHENYLALANINE'>PHE</scene></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phenylalanine--tRNA_ligase Phenylalanine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.20 6.1.1.20] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3l4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l4g OCA], [https://pdbe.org/3l4g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3l4g RCSB], [https://www.ebi.ac.uk/pdbsum/3l4g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3l4g ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3l4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l4g OCA], [http://pdbe.org/3l4g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3l4g RCSB], [http://www.ebi.ac.uk/pdbsum/3l4g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3l4g ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/SYFA_HUMAN SYFA_HUMAN]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 30: / Line 31: @@
 ==See Also==
-*[[Aminoacyl tRNA Synthetase|Aminoacyl tRNA Synthetase]]
+*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
-[[Category: Phenylalanine--tRNA ligase]]
+[[Category: Large Structures]]
-[[Category: Finarov, I]]
+[[Category: Finarov I]]
-[[Category: Kessler, N]]
+[[Category: Kessler N]]
-[[Category: Klipcan, L]]
+[[Category: Klipcan L]]
-[[Category: Moor, N]]
+[[Category: Moor N]]
-[[Category: Safro, M G]]
+[[Category: Safro MG]]
-[[Category: Acetylation]]
-[[Category: Aminoacyl-trna synthetase]]
-[[Category: Aminoacylation]]
-[[Category: Atp-binding]]
-[[Category: Cytoplasm]]
-[[Category: Dna-binding domain]]
-[[Category: Four-helix bundle]]
-[[Category: Ligase]]
-[[Category: Nucleotide-binding]]
-[[Category: Phosphoprotein]]
-[[Category: Polymorphism]]
-[[Category: Protein biosynthesis]]
-[[Category: Trna-binding]]

3l4g

From Proteopedia

Current revision

Crystal structure of Homo Sapiens cytoplasmic Phenylalanyl-tRNA synthetase

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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