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| | ==Structure of Aristaless homeodomain in complex with DNA== | | ==Structure of Aristaless homeodomain in complex with DNA== |
| - | <StructureSection load='3lnq' size='340' side='right' caption='[[3lnq]], [[Resolution|resolution]] 2.25Å' scene=''> | + | <StructureSection load='3lnq' size='340' side='right'caption='[[3lnq]], [[Resolution|resolution]] 2.25Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3lnq]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LNQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LNQ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3lnq]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LNQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LNQ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">al, CG3935 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3lnq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lnq OCA], [http://pdbe.org/3lnq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3lnq RCSB], [http://www.ebi.ac.uk/pdbsum/3lnq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3lnq ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3lnq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lnq OCA], [https://pdbe.org/3lnq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3lnq RCSB], [https://www.ebi.ac.uk/pdbsum/3lnq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3lnq ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/AL_DROME AL_DROME]] Involved in the morphogenesis of proximal and distal pattern elements in a subset of appendages. Also has a role in early imaginal disk development. | + | [https://www.uniprot.org/uniprot/AL_DROME AL_DROME] Involved in the morphogenesis of proximal and distal pattern elements in a subset of appendages. Also has a role in early imaginal disk development. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Drome]] | + | [[Category: Drosophila melanogaster]] |
| - | [[Category: Kojima, T]] | + | [[Category: Large Structures]] |
| - | [[Category: Miyazono, K]] | + | [[Category: Kojima T]] |
| - | [[Category: Nagata, K]] | + | [[Category: Miyazono K]] |
| - | [[Category: Saigo, K]] | + | [[Category: Nagata K]] |
| - | [[Category: Takamura, Y]] | + | [[Category: Saigo K]] |
| - | [[Category: Tanokura, M]] | + | [[Category: Takamura Y]] |
| - | [[Category: Developmental protein]]
| + | [[Category: Tanokura M]] |
| - | [[Category: Dna-binding]]
| + | |
| - | [[Category: Gene regulation-dna complex]]
| + | |
| - | [[Category: Homeobox]]
| + | |
| - | [[Category: Homeodomain]]
| + | |
| - | [[Category: Nucleus]]
| + | |
| - | [[Category: Protein-dna complex]]
| + | |
| Structural highlights
Function
AL_DROME Involved in the morphogenesis of proximal and distal pattern elements in a subset of appendages. Also has a role in early imaginal disk development.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
To achieve accurate gene regulation, some homeodomain proteins bind cooperatively to DNA to increase those site specificities. We report a ternary complex structure containing two homeodomain proteins, aristaless (Al) and clawless (Cll), bound to DNA. Our results show that the extended conserved sequences of the Cll homeodomain are indispensable to cooperative DNA binding. In the Al-Cll-DNA complex structure, the residues in the extended regions are used not only for the intermolecular contacts between the two homeodomain proteins but also for the sequence-recognition mechanism of DNA by direct interactions. The residues in the extended N-terminal arm lie within the minor groove of DNA to form direct interactions with bases, whereas the extended conserved region of the C-terminus of the homeodomain interacts with Al to stabilize and localize the third alpha helix of the Cll homeodomain. This structure suggests a novel mode for the cooperativity of homeodomain proteins.
Cooperative DNA-binding and sequence-recognition mechanism of aristaless and clawless.,Miyazono K, Zhi Y, Takamura Y, Nagata K, Saigo K, Kojima T, Tanokura M EMBO J. 2010 May 5;29(9):1613-23. Epub 2010 Apr 13. PMID:20389279[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Miyazono K, Zhi Y, Takamura Y, Nagata K, Saigo K, Kojima T, Tanokura M. Cooperative DNA-binding and sequence-recognition mechanism of aristaless and clawless. EMBO J. 2010 May 5;29(9):1613-23. Epub 2010 Apr 13. PMID:20389279 doi:10.1038/emboj.2010.53
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