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| | <StructureSection load='3nij' size='340' side='right'caption='[[3nij]], [[Resolution|resolution]] 2.10Å' scene=''> | | <StructureSection load='3nij' size='340' side='right'caption='[[3nij]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3nij]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NIJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NIJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3nij]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NIJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NIJ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3nih|3nih]], [[3nii|3nii]], [[3nik|3nik]], [[3nil|3nil]], [[3nim|3nim]], [[3nin|3nin]], [[3nis|3nis]], [[3nit|3nit]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nij OCA], [http://pdbe.org/3nij PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3nij RCSB], [http://www.ebi.ac.uk/pdbsum/3nij PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3nij ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nij OCA], [https://pdbe.org/3nij PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nij RCSB], [https://www.ebi.ac.uk/pdbsum/3nij PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nij ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/UBR1_YEAST UBR1_YEAST]] Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. | + | [https://www.uniprot.org/uniprot/UBR1_YEAST UBR1_YEAST] Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | ==See Also== | | ==See Also== |
| - | *[[Ubiquitin protein ligase|Ubiquitin protein ligase]] | + | *[[Ubiquitin protein ligase 3D structures|Ubiquitin protein ligase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 18824]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Choi, W S]] | + | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: Jeong, B C]] | + | [[Category: Choi WS]] |
| - | [[Category: Lee, M R]] | + | [[Category: Jeong B-C]] |
| - | [[Category: Song, H K]] | + | [[Category: Lee M-R]] |
| - | [[Category: E3 ubiquitin ligase]]
| + | [[Category: Song HK]] |
| - | [[Category: Ligase]]
| + | |
| - | [[Category: Metal binding protein]]
| + | |
| - | [[Category: N-end rule]]
| + | |
| - | [[Category: Ubr box]]
| + | |
| - | [[Category: Zinc-binding protein]]
| + | |
| Structural highlights
Function
UBR1_YEAST Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The N-end rule pathway is a regulated proteolytic system that targets proteins containing destabilizing N-terminal residues (N-degrons) for ubiquitination and proteasomal degradation in eukaryotes. The N-degrons of type 1 substrates contain an N-terminal basic residue that is recognized by the UBR box domain of the E3 ubiquitin ligase UBR1. We describe structures of the UBR box of Saccharomyces cerevisiae UBR1 alone and in complex with N-degron peptides, including that of the cohesin subunit Scc1, which is cleaved and targeted for degradation at the metaphase-anaphase transition. The structures reveal a previously unknown protein fold that is stabilized by a novel binuclear zinc center. N-terminal arginine, lysine or histidine side chains of the N-degron are coordinated in a multispecific binding pocket. Unexpectedly, the structures together with our in vitro biochemical and in vivo pulse-chase analyses reveal a previously unknown modulation of binding specificity by the residue at position 2 of the N-degron.
Structural basis for the recognition of N-end rule substrates by the UBR box of ubiquitin ligases.,Choi WS, Jeong BC, Joo YJ, Lee MR, Kim J, Eck MJ, Song HK Nat Struct Mol Biol. 2010 Sep 12. PMID:20835240[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Choi WS, Jeong BC, Joo YJ, Lee MR, Kim J, Eck MJ, Song HK. Structural basis for the recognition of N-end rule substrates by the UBR box of ubiquitin ligases. Nat Struct Mol Biol. 2010 Sep 12. PMID:20835240 doi:10.1038/nsmb.1907
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