1ow3

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Revision as of 16:30, 12 November 2007

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spinqualitylabelsall models 1ow3, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal Structure of RhoA.GDP.MgF3-in Complex with RhoGAP

Overview

The formation of complexes between small G proteins and certain of their, effectors can be facilitated by aluminum fluorides. Solution studies, suggest that magnesium may be able to replace aluminum in such complexes., We have determined the crystal structure of RhoA.GDP bound to RhoGAP in, the presence of Mg(2+) and F(-) but without Al(3+). The metallofluoride, adopts a trigonal planar arrangement instead of the square planar, structure of AlF(4)(-). We have confirmed that these crystals contain, magnesium and not aluminum by proton-induced X-ray emission spectroscopy., The structure adopted by GDP.MgF(-) possesses the stereochemistry and, approximate charge expected for the transition state. We suggest that, MgF3(-) may be the reagent of choice for studying phosphoryl transfer, reactions.

About this Structure

1OW3 is a Protein complex structure of sequences from Homo sapiens with MG, GDP and MGF as ligands. Full crystallographic information is available from OCA.

Reference

MgF(3)(-) as a transition state analog of phosphoryl transfer., Graham DL, Lowe PN, Grime GW, Marsh M, Rittinger K, Smerdon SJ, Gamblin SJ, Eccleston JF, Chem Biol. 2002 Mar;9(3):375-81. PMID:11927263

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