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1nzs
From Proteopedia
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[[Image:1nzs.jpg|left|200px]] | [[Image:1nzs.jpg|left|200px]] | ||
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'''NMR structures of phosphorylated carboxy terminus of bovine rhodopsin in arrestin-bound state''' | '''NMR structures of phosphorylated carboxy terminus of bovine rhodopsin in arrestin-bound state''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1NZS is a [[Single protein]] structure | + | 1NZS is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NZS OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Kisselev, O G.]] | [[Category: Kisselev, O G.]] | ||
[[Category: McDowell, J H.]] | [[Category: McDowell, J H.]] | ||
| - | [[Category: | + | [[Category: Arrestin]] |
| - | [[Category: | + | [[Category: Ball-and-chain]] |
| - | [[Category: | + | [[Category: Bound conformation]] |
| - | [[Category: | + | [[Category: Gpcr]] |
| - | [[Category: | + | [[Category: Helix-loop]] |
| - | [[Category: | + | [[Category: Phosphorylation]] |
| - | [[Category: | + | [[Category: Rhodopsin]] |
| - | [[Category: | + | [[Category: Signal termination]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:11:23 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 00:11, 3 May 2008
NMR structures of phosphorylated carboxy terminus of bovine rhodopsin in arrestin-bound state
Overview
Visual arrestin binds to the phosphorylated carboxy-terminal region of rhodopsin to block interactions with transducin and terminate signaling in the rod photoreceptor cells. A synthetic seven-phospho-peptide from the C-terminal region of rhodopsin, Rh(330-348), has been shown to bind arrestin and mimic inhibition of signal transduction. In this study, we examine conformational changes in this synthetic peptide upon binding to arrestin by high-resolution proton nuclear magnetic resonance (NMR). We show that the peptide is completely disordered in solution, but becomes structured upon binding to arrestin. A control, unphosphorylated peptide that fails to bind to arrestin remains highly disordered. Specific NMR distance constraints are used to model the arrestin-bound conformation. The models suggest that the phosphorylated carboxy-terminal region of rhodopsin, Rh(330-348), undergoes significant conformational changes and becomes structured upon binding to arrestin.
About this Structure
1NZS is a Single protein structure. Full crystallographic information is available from OCA.
Reference
The arrestin-bound conformation and dynamics of the phosphorylated carboxy-terminal region of rhodopsin., Kisselev OG, McDowell JH, Hargrave PA, FEBS Lett. 2004 Apr 30;564(3):307-11. PMID:15111114 Page seeded by OCA on Sat May 3 03:11:23 2008
