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| | ==Crystal structure of human ACBD3 GOLD domain in complex with 3A protein of Aichivirus B== | | ==Crystal structure of human ACBD3 GOLD domain in complex with 3A protein of Aichivirus B== |
| - | <StructureSection load='5lz6' size='340' side='right' caption='[[5lz6]], [[Resolution|resolution]] 2.60Å' scene=''> | + | <StructureSection load='5lz6' size='340' side='right'caption='[[5lz6]], [[Resolution|resolution]] 2.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5lz6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aichivirus_b Aichivirus b] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LZ6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LZ6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5lz6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aichivirus_B Aichivirus B] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LZ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LZ6 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ACBD3, GCP60, GOCAP1, GOLPH1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lz6 OCA], [http://pdbe.org/5lz6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lz6 RCSB], [http://www.ebi.ac.uk/pdbsum/5lz6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lz6 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lz6 OCA], [https://pdbe.org/5lz6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lz6 RCSB], [https://www.ebi.ac.uk/pdbsum/5lz6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lz6 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/GCP60_HUMAN GCP60_HUMAN]] Involved in the maintenance of Golgi structure by interacting with giantin, affecting protein transport between the endoplasmic reticulum and Golgi. Involved in hormone-induced steroid biosynthesis in testicular Leydig cells (By similarity).<ref>PMID:11590181</ref> | + | [https://www.uniprot.org/uniprot/GCP60_HUMAN GCP60_HUMAN] Involved in the maintenance of Golgi structure by interacting with giantin, affecting protein transport between the endoplasmic reticulum and Golgi. Involved in hormone-induced steroid biosynthesis in testicular Leydig cells (By similarity).<ref>PMID:11590181</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Aichivirus b]] | + | [[Category: Aichivirus B]] |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Boura, E]] | + | [[Category: Large Structures]] |
| - | [[Category: Klima, M]] | + | [[Category: Boura E]] |
| - | [[Category: Acbd3]] | + | [[Category: Klima M]] |
| - | [[Category: Aichivirus]]
| + | |
| - | [[Category: Antiviral protein]]
| + | |
| - | [[Category: Gold]]
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| Structural highlights
Function
GCP60_HUMAN Involved in the maintenance of Golgi structure by interacting with giantin, affecting protein transport between the endoplasmic reticulum and Golgi. Involved in hormone-induced steroid biosynthesis in testicular Leydig cells (By similarity).[1]
Publication Abstract from PubMed
Picornaviruses are small positive-sense single-stranded RNA viruses that include many important human pathogens. Within the host cell, they replicate at specific replication sites called replication organelles. To create this membrane platform, they hijack several host factors including the acyl-CoA-binding domain-containing protein-3 (ACBD3). Here, we present a structural characterization of the molecular complexes formed by the non-structural 3A proteins from two species of the Kobuvirus genus of the Picornaviridae family and the 3A-binding domain of the host ACBD3 protein. Specifically, we present a series of crystal structures as well as a molecular dynamics simulation of the 3A:ACBD3 complex at the membrane, which reveals that the viral 3A proteins act as molecular harnesses to enslave the ACBD3 protein leading to its stabilization at target membranes. Our data provide a structural rationale for understanding how these viral-host protein complexes assemble at the atomic level and identify new potential targets for antiviral therapies.
Kobuviral Non-structural 3A Proteins Act as Molecular Harnesses to Hijack the Host ACBD3 Protein.,Klima M, Chalupska D, Rozycki B, Humpolickova J, Rezabkova L, Silhan J, Baumlova A, Dubankova A, Boura E Structure. 2017 Feb 7;25(2):219-230. doi: 10.1016/j.str.2016.11.021. Epub 2017, Jan 5. PMID:28065508[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Sohda M, Misumi Y, Yamamoto A, Yano A, Nakamura N, Ikehara Y. Identification and characterization of a novel Golgi protein, GCP60, that interacts with the integral membrane protein giantin. J Biol Chem. 2001 Nov 30;276(48):45298-306. Epub 2001 Oct 5. PMID:11590181 doi:http://dx.doi.org/10.1074/jbc.M108961200
- ↑ Klima M, Chalupska D, Rozycki B, Humpolickova J, Rezabkova L, Silhan J, Baumlova A, Dubankova A, Boura E. Kobuviral Non-structural 3A Proteins Act as Molecular Harnesses to Hijack the Host ACBD3 Protein. Structure. 2017 Feb 7;25(2):219-230. doi: 10.1016/j.str.2016.11.021. Epub 2017, Jan 5. PMID:28065508 doi:http://dx.doi.org/10.1016/j.str.2016.11.021
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