5m04

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<StructureSection load='5m04' size='340' side='right'caption='[[5m04]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
<StructureSection load='5m04' size='340' side='right'caption='[[5m04]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5m04]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_dh5[alpha] Escherichia coli dh5[alpha]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M04 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5M04 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5m04]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_DH5alpha Escherichia coli DH5alpha]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M04 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5M04 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">obgE, cgtA, obg, yhbZ, b3183, JW3150 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=668369 Escherichia coli DH5[alpha]])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5m04 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m04 OCA], [http://pdbe.org/5m04 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5m04 RCSB], [http://www.ebi.ac.uk/pdbsum/5m04 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5m04 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5m04 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m04 OCA], [https://pdbe.org/5m04 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5m04 RCSB], [https://www.ebi.ac.uk/pdbsum/5m04 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5m04 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/OBG_ECOLI OBG_ECOLI]] An abundant, essential GTPase which binds GTP, GDP and ppGpp with moderate affinity, shows high guanine nucleotide exchange rate constants for GTP and GDP, and has a relatively low GTP hydrolysis rate. It is estimated there are 34000 molecules in log-phase cells and 5600 molecules in stationary-phase cells as the monomer. Required for chromosome segregation. It also plays a role in the stringent response, and a non-essential role in the late steps of ribosome biogenesis, but how it performs these functions remains unelucidated.<ref>PMID:11555285</ref> <ref>PMID:12402086</ref> <ref>PMID:11976298</ref> <ref>PMID:12855728</ref> <ref>PMID:12826057</ref> <ref>PMID:15836769</ref> <ref>PMID:15721258</ref> <ref>PMID:16518617</ref> <ref>PMID:16980477</ref> <ref>PMID:17616600</ref> <ref>PMID:17578452</ref> <ref>PMID:19555460</ref> Required for correct chromosome partitioning; in temperature-sensitive (ts) mutant nucleoids do not partition but remain in the middle of cell, cells elongate but do not divide. Overexpression protects cells against UV damage. Ts mutants have impaired plasmid and lamdba phage replication, possibly via effects on DnaA. Regulates DnaA levels. Genetic interactions of Val-168 and a C-terminal insertion mutant with the double-strand break repair factors recA and recBCD, and with seqA suggests that ObgE, either directly or indirectly, promotes replication fork stability. Initiation of DNA replication continues in ObgE-depleted cells.<ref>PMID:11555285</ref> <ref>PMID:12402086</ref> <ref>PMID:11976298</ref> <ref>PMID:12855728</ref> <ref>PMID:12826057</ref> <ref>PMID:15836769</ref> <ref>PMID:15721258</ref> <ref>PMID:16518617</ref> <ref>PMID:16980477</ref> <ref>PMID:17616600</ref> <ref>PMID:17578452</ref> <ref>PMID:19555460</ref> Binds to pre-50S ribosomal subunits in a salt-dependent manner, has also been seen associated with 30S subunits. Overexpression rescues an rrmJ deletion stabilizing the 70S ribosome. Even at permissive temperatures the ts mutant (Gln-80/Asn-85) shows disrupted 50S ribosomal subunit assembly, defects in 16 and 23S rRNA processing and altered association of some late-assembling ribosomal proteins. Dissociates from the pre-50S ribosome under conditions of amino acid starvation. The levels of (p)ppGpp rise in the ts mutant (Gln-80/Asn-85), possibly because ObgE controls SpoT. Binds GDP and ppGpp with the same affinity. During ribosome assembly ObgE acts later than the rRNA methyltransferase rrmj and DEAD-box RNA helicases DeaD and SrmB.<ref>PMID:11555285</ref> <ref>PMID:12402086</ref> <ref>PMID:11976298</ref> <ref>PMID:12855728</ref> <ref>PMID:12826057</ref> <ref>PMID:15836769</ref> <ref>PMID:15721258</ref> <ref>PMID:16518617</ref> <ref>PMID:16980477</ref> <ref>PMID:17616600</ref> <ref>PMID:17578452</ref> <ref>PMID:19555460</ref>
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[https://www.uniprot.org/uniprot/OBG_ECOLI OBG_ECOLI] An abundant, essential GTPase which binds GTP, GDP and ppGpp with moderate affinity, shows high guanine nucleotide exchange rate constants for GTP and GDP, and has a relatively low GTP hydrolysis rate. It is estimated there are 34000 molecules in log-phase cells and 5600 molecules in stationary-phase cells as the monomer. Required for chromosome segregation. It also plays a role in the stringent response, and a non-essential role in the late steps of ribosome biogenesis, but how it performs these functions remains unelucidated.<ref>PMID:11555285</ref> <ref>PMID:12402086</ref> <ref>PMID:11976298</ref> <ref>PMID:12855728</ref> <ref>PMID:12826057</ref> <ref>PMID:15836769</ref> <ref>PMID:15721258</ref> <ref>PMID:16518617</ref> <ref>PMID:16980477</ref> <ref>PMID:17616600</ref> <ref>PMID:17578452</ref> <ref>PMID:19555460</ref> Required for correct chromosome partitioning; in temperature-sensitive (ts) mutant nucleoids do not partition but remain in the middle of cell, cells elongate but do not divide. Overexpression protects cells against UV damage. Ts mutants have impaired plasmid and lamdba phage replication, possibly via effects on DnaA. Regulates DnaA levels. Genetic interactions of Val-168 and a C-terminal insertion mutant with the double-strand break repair factors recA and recBCD, and with seqA suggests that ObgE, either directly or indirectly, promotes replication fork stability. Initiation of DNA replication continues in ObgE-depleted cells.<ref>PMID:11555285</ref> <ref>PMID:12402086</ref> <ref>PMID:11976298</ref> <ref>PMID:12855728</ref> <ref>PMID:12826057</ref> <ref>PMID:15836769</ref> <ref>PMID:15721258</ref> <ref>PMID:16518617</ref> <ref>PMID:16980477</ref> <ref>PMID:17616600</ref> <ref>PMID:17578452</ref> <ref>PMID:19555460</ref> Binds to pre-50S ribosomal subunits in a salt-dependent manner, has also been seen associated with 30S subunits. Overexpression rescues an rrmJ deletion stabilizing the 70S ribosome. Even at permissive temperatures the ts mutant (Gln-80/Asn-85) shows disrupted 50S ribosomal subunit assembly, defects in 16 and 23S rRNA processing and altered association of some late-assembling ribosomal proteins. Dissociates from the pre-50S ribosome under conditions of amino acid starvation. The levels of (p)ppGpp rise in the ts mutant (Gln-80/Asn-85), possibly because ObgE controls SpoT. Binds GDP and ppGpp with the same affinity. During ribosome assembly ObgE acts later than the rRNA methyltransferase rrmj and DEAD-box RNA helicases DeaD and SrmB.<ref>PMID:11555285</ref> <ref>PMID:12402086</ref> <ref>PMID:11976298</ref> <ref>PMID:12855728</ref> <ref>PMID:12826057</ref> <ref>PMID:15836769</ref> <ref>PMID:15721258</ref> <ref>PMID:16518617</ref> <ref>PMID:16980477</ref> <ref>PMID:17616600</ref> <ref>PMID:17578452</ref> <ref>PMID:19555460</ref>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Gkekas, S]]
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[[Category: Gkekas S]]
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[[Category: Singh, R K]]
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[[Category: Singh RK]]
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[[Category: Versees, W]]
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[[Category: Versees W]]
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[[Category: Cgta]]
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[[Category: Gtpase]]
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[[Category: Hydrolase]]
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[[Category: Obge]]
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Current revision

Structure of ObgE from Escherichia coli

PDB ID 5m04

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