1o08

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[[Image:1o08.gif|left|200px]]
[[Image:1o08.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1o08 |SIZE=350|CAPTION= <scene name='initialview01'>1o08</scene>, resolution 1.20&Aring;
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The line below this paragraph, containing "STRUCTURE_1o08", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=G16:ALPHA-D-GLUCOSE+1,6-BISPHOSPHATE'>G16</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-phosphoglucomutase Beta-phosphoglucomutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.2.6 5.4.2.6] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1o08| PDB=1o08 | SCENE= }}
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|RELATEDENTRY=[[1lvh|1LVH]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o08 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o08 OCA], [http://www.ebi.ac.uk/pdbsum/1o08 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1o08 RCSB]</span>
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}}
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'''Structure of Pentavalent Phosphorous Intermediate of an Enzyme Catalyzed Phosphoryl transfer Reaction observed on cocrystallization with Glucose 1-phosphate'''
'''Structure of Pentavalent Phosphorous Intermediate of an Enzyme Catalyzed Phosphoryl transfer Reaction observed on cocrystallization with Glucose 1-phosphate'''
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[[Category: Lahiri, S D.]]
[[Category: Lahiri, S D.]]
[[Category: Zhang, G.]]
[[Category: Zhang, G.]]
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[[Category: haloacid dehalogenase superfamily]]
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[[Category: Haloacid dehalogenase superfamily]]
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[[Category: pentavalent phosphate intermediate]]
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[[Category: Pentavalent phosphate intermediate]]
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[[Category: phosphotransferase]]
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[[Category: Phosphotransferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:12:11 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:38:00 2008''
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Revision as of 00:12, 3 May 2008

Image:1o08.gif

Template:STRUCTURE 1o08

Structure of Pentavalent Phosphorous Intermediate of an Enzyme Catalyzed Phosphoryl transfer Reaction observed on cocrystallization with Glucose 1-phosphate


Overview

Enzymes provide enormous rate enhancements, unmatched by any other type of catalyst. The stabilization of high-energy states along the reaction coordinate is the crux of the catalytic power of enzymes. We report the atomic-resolution structure of a high-energy reaction intermediate stabilized in the active site of an enzyme. Crystallization of phosphorylated beta-phosphoglucomutase in the presence of the Mg(II) cofactor and either of the substrates glucose 1-phosphate or glucose 6-phosphate produced crystals of the enzyme-Mg(II)-glucose 1,6-(bis)phosphate complex, which diffracted x-rays to 1.2 and 1.4 angstroms, respectively. The structure reveals a stabilized pentacovalent phosphorane formed in the phosphoryl transfer from the C(1)O of glucose 1,6-(bis)phosphate to the nucleophilic Asp8 carboxylate.

About this Structure

1O08 is a Single protein structure of sequence from Lactococcus lactis. Full crystallographic information is available from OCA.

Reference

The pentacovalent phosphorus intermediate of a phosphoryl transfer reaction., Lahiri SD, Zhang G, Dunaway-Mariano D, Allen KN, Science. 2003 Mar 28;299(5615):2067-71. Epub 2003 Mar 13. PMID:12637673 Page seeded by OCA on Sat May 3 03:12:11 2008

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