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| | <StructureSection load='5m3e' size='340' side='right'caption='[[5m3e]], [[Resolution|resolution]] 2.50Å' scene=''> | | <StructureSection load='5m3e' size='340' side='right'caption='[[5m3e]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5m3e]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus_y51mc23 Thermus aquaticus y51mc23]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M3E OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5M3E FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5m3e]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_aquaticus_Y51MC23 Thermus aquaticus Y51MC23]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M3E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5M3E FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=APR:ADENOSINE-5-DIPHOSPHORIBOSE'>APR</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TaqDRAFT_4250 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=498848 Thermus aquaticus Y51MC23])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=APR:ADENOSINE-5-DIPHOSPHORIBOSE'>APR</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5m3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m3e OCA], [http://pdbe.org/5m3e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5m3e RCSB], [http://www.ebi.ac.uk/pdbsum/5m3e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5m3e ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5m3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m3e OCA], [https://pdbe.org/5m3e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5m3e RCSB], [https://www.ebi.ac.uk/pdbsum/5m3e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5m3e ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/DARG_THEA5 DARG_THEA5] Antitoxin component of a hybrid type II/IV toxin-antitoxin (TA) system. De-ADP-ribosylates DNA modified on thymidine by its cognate toxin DarT, which neutralizes the activity of cognate toxin DarT. Upon expression in E.coli neutralizes the effect of cognate toxin DarT (PubMed:27939941). Upon expression in M.tuberculosis neutralizes the toxic effects of endogenous DarT (PubMed:32634279).<ref>PMID:27939941</ref> <ref>PMID:32634279</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Thermus aquaticus y51mc23]] | + | [[Category: Thermus aquaticus Y51MC23]] |
| - | [[Category: Ariza, A]] | + | [[Category: Ariza A]] |
| - | [[Category: Adp-ribose]]
| + | |
| - | [[Category: Adp-ribosylation]]
| + | |
| - | [[Category: Antitoxin]]
| + | |
| - | [[Category: Macrodomain]]
| + | |
| - | [[Category: Toxin-antitoxin]]
| + | |
| Structural highlights
Function
DARG_THEA5 Antitoxin component of a hybrid type II/IV toxin-antitoxin (TA) system. De-ADP-ribosylates DNA modified on thymidine by its cognate toxin DarT, which neutralizes the activity of cognate toxin DarT. Upon expression in E.coli neutralizes the effect of cognate toxin DarT (PubMed:27939941). Upon expression in M.tuberculosis neutralizes the toxic effects of endogenous DarT (PubMed:32634279).[1] [2]
Publication Abstract from PubMed
The discovery and study of toxin-antitoxin (TA) systems helps us advance our understanding of the strategies prokaryotes employ to regulate cellular processes related to the general stress response, such as defense against phages, growth control, biofilm formation, persistence, and programmed cell death. Here we identify and characterize a TA system found in various bacteria, including the global pathogen Mycobacterium tuberculosis. The toxin of the system (DarT) is a domain of unknown function (DUF) 4433, and the antitoxin (DarG) a macrodomain protein. We demonstrate that DarT is an enzyme that specifically modifies thymidines on single-stranded DNA in a sequence-specific manner by a nucleotide-type modification called ADP-ribosylation. We also show that this modification can be removed by DarG. Our results provide an example of reversible DNA ADP-ribosylation, and we anticipate potential therapeutic benefits by targeting this enzyme-enzyme TA system in bacterial pathogens such as M. tuberculosis.
The Toxin-Antitoxin System DarTG Catalyzes Reversible ADP-Ribosylation of DNA.,Jankevicius G, Ariza A, Ahel M, Ahel I Mol Cell. 2016 Dec 15;64(6):1109-1116. doi: 10.1016/j.molcel.2016.11.014. Epub, 2016 Dec 8. PMID:27939941[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Jankevicius G, Ariza A, Ahel M, Ahel I. The Toxin-Antitoxin System DarTG Catalyzes Reversible ADP-Ribosylation of DNA. Mol Cell. 2016 Dec 15;64(6):1109-1116. doi: 10.1016/j.molcel.2016.11.014. Epub, 2016 Dec 8. PMID:27939941 doi:http://dx.doi.org/10.1016/j.molcel.2016.11.014
- ↑ Zaveri A, Wang R, Botella L, Sharma R, Zhu L, Wallach JB, Song N, Jansen RS, Rhee KY, Ehrt S, Schnappinger D. Depletion of the DarG antitoxin in Mycobacterium tuberculosis triggers the DNA-damage response and leads to cell death. Mol Microbiol. 2020 Oct;114(4):641-652. PMID:32634279 doi:10.1111/mmi.14571
- ↑ Jankevicius G, Ariza A, Ahel M, Ahel I. The Toxin-Antitoxin System DarTG Catalyzes Reversible ADP-Ribosylation of DNA. Mol Cell. 2016 Dec 15;64(6):1109-1116. doi: 10.1016/j.molcel.2016.11.014. Epub, 2016 Dec 8. PMID:27939941 doi:http://dx.doi.org/10.1016/j.molcel.2016.11.014
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