|
|
| Line 3: |
Line 3: |
| | <StructureSection load='5m4y' size='340' side='right'caption='[[5m4y]], [[Resolution|resolution]] 2.20Å' scene=''> | | <StructureSection load='5m4y' size='340' side='right'caption='[[5m4y]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5m4y]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M4Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5M4Y FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5m4y]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M4Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5M4Y FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SSO2, YMR183C, YM8010.13C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), SEC3, PSL1, YER008C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5m4y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m4y OCA], [http://pdbe.org/5m4y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5m4y RCSB], [http://www.ebi.ac.uk/pdbsum/5m4y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5m4y ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5m4y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m4y OCA], [https://pdbe.org/5m4y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5m4y RCSB], [https://www.ebi.ac.uk/pdbsum/5m4y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5m4y ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SSO2_YEAST SSO2_YEAST]] Required for vesicle fusion with the plasma membrane. [[http://www.uniprot.org/uniprot/SEC3_YEAST SEC3_YEAST]] Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. | + | [https://www.uniprot.org/uniprot/SSO2_YEAST SSO2_YEAST] Required for vesicle fusion with the plasma membrane. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 23: |
Line 23: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Dong, G]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Zhang, Y B]] | + | [[Category: Dong G]] |
| - | [[Category: Exocyst]] | + | [[Category: Zhang YB]] |
| - | [[Category: Sec3]]
| + | |
| - | [[Category: Sso2]]
| + | |
| - | [[Category: Structural protein]]
| + | |
| Structural highlights
Function
SSO2_YEAST Required for vesicle fusion with the plasma membrane.
Publication Abstract from PubMed
The soluble N-ethylmaleimide-sensitive factor-attachment protein receptors (SNAREs) constitute the core machinery for membrane fusion during eukaryotic cell vesicular trafficking. However, how the assembly of the SNARE complex is initiated is unknown. Here we report that Sec3, a component of the exocyst complex that mediates vesicle tethering during exocytosis, directly interacts with the t-SNARE protein Sso2. This interaction promotes the formation of an Sso2-Sec9 'binary' t-SNARE complex, the early rate-limiting step in SNARE complex assembly, and stimulates membrane fusion. The crystal structure of the Sec3-Sso2 complex suggests that Sec3 binding induces conformational changes of Sso2 that are crucial for the relief of its auto-inhibition. Interestingly, specific disruption of the Sec3-Sso2 interaction in cells blocks exocytosis without affecting the function of Sec3 in vesicle tethering. Our study reveals an activation mechanism for SNARE complex assembly, and uncovers a role of the exocyst in promoting membrane fusion in addition to vesicle tethering.
Sec3 promotes the initial binary t-SNARE complex assembly and membrane fusion.,Yue P, Zhang Y, Mei K, Wang S, Lesigang J, Zhu Y, Dong G, Guo W Nat Commun. 2017 Jan 23;8:14236. doi: 10.1038/ncomms14236. PMID:28112172[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Yue P, Zhang Y, Mei K, Wang S, Lesigang J, Zhu Y, Dong G, Guo W. Sec3 promotes the initial binary t-SNARE complex assembly and membrane fusion. Nat Commun. 2017 Jan 23;8:14236. doi: 10.1038/ncomms14236. PMID:28112172 doi:http://dx.doi.org/10.1038/ncomms14236
|
