|
|
| Line 1: |
Line 1: |
| | | | |
| | ==Crystal structure of the flagellin-FliS complex from Bacillus subtilis== | | ==Crystal structure of the flagellin-FliS complex from Bacillus subtilis== |
| - | <StructureSection load='5maw' size='340' side='right' caption='[[5maw]], [[Resolution|resolution]] 1.50Å' scene=''> | + | <StructureSection load='5maw' size='340' side='right'caption='[[5maw]], [[Resolution|resolution]] 1.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5maw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MAW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MAW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5maw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MAW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MAW FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hag, BSU35360 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835]), fliS, BSU35330 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5maw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5maw OCA], [http://pdbe.org/5maw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5maw RCSB], [http://www.ebi.ac.uk/pdbsum/5maw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5maw ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5maw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5maw OCA], [https://pdbe.org/5maw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5maw RCSB], [https://www.ebi.ac.uk/pdbsum/5maw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5maw ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/FLA_BACSU FLA_BACSU]] Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. | + | [https://www.uniprot.org/uniprot/FLA_BACSU FLA_BACSU] Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 20: |
Line 20: |
| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Flagellar protein|Flagellar protein]] | + | *[[Flagellar protein 3D structures|Flagellar protein 3D structures]] |
| | + | *[[Flagellin 3D structures|Flagellin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Vibrio subtilis ehrenberg 1835]] | + | [[Category: Bacillus subtilis]] |
| - | [[Category: Altegoer, F]] | + | [[Category: Large Structures]] |
| - | [[Category: Bange, G]] | + | [[Category: Altegoer F]] |
| - | [[Category: Chaperone]] | + | [[Category: Bange G]] |
| - | [[Category: Flagellum]]
| + | |
| - | [[Category: Type-3-secretion]]
| + | |
| Structural highlights
Function
FLA_BACSU Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.
Publication Abstract from PubMed
Flagellin is amongst the most abundant proteins in flagellated bacterial species and constitutes the major building block of the flagellar filament. The proteins FliW and FliS serve in the post-transcriptional control of flagellin and guide the protein to the flagellar type III secretion system (fT3SS), respectively. Here, we present the high-resolution structure of FliS/flagellin heterodimer and show that FliS and FliW bind to opposing interfaces located at the N- and C-termini of flagellin. The FliS/flagellin/FliW heterotrimer is able to interact with FlhA-C suggesting that FliW and FliS are released during flagellin export. After release, FliW and FliS are recycled to execute a new round of post-transcriptional regulation and targeting. Taken together, our study provides a mechanism explaining how FliW and FliS synchronize the production of flagellin with the capacity of the fT3SS to secrete flagellin.
FliS/flagellin/FliW heterotrimer couples type III secretion and flagellin homeostasis.,Altegoer F, Mukherjee S, Steinchen W, Bedrunka P, Linne U, Kearns DB, Bange G Sci Rep. 2018 Aug 1;8(1):11552. doi: 10.1038/s41598-018-29884-8. PMID:30068950[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Altegoer F, Mukherjee S, Steinchen W, Bedrunka P, Linne U, Kearns DB, Bange G. FliS/flagellin/FliW heterotrimer couples type III secretion and flagellin homeostasis. Sci Rep. 2018 Aug 1;8(1):11552. doi: 10.1038/s41598-018-29884-8. PMID:30068950 doi:http://dx.doi.org/10.1038/s41598-018-29884-8
|
