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| | ==Crystal structure of in vitro folded Chitoporin VhChip from Vibrio harveyi (crystal form II)== | | ==Crystal structure of in vitro folded Chitoporin VhChip from Vibrio harveyi (crystal form II)== |
| - | <StructureSection load='5mdp' size='340' side='right' caption='[[5mdp]], [[Resolution|resolution]] 3.08Å' scene=''> | + | <StructureSection load='5mdp' size='340' side='right'caption='[[5mdp]], [[Resolution|resolution]] 3.08Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5mdp]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"achromobacter_harveyi"_johnson_and_shunk_1936 "achromobacter harveyi" johnson and shunk 1936]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MDP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MDP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5mdp]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_harveyi Vibrio harveyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MDP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MDP FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.08Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">chiP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=669 "Achromobacter harveyi" Johnson and Shunk 1936])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mdp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mdp OCA], [http://pdbe.org/5mdp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mdp RCSB], [http://www.ebi.ac.uk/pdbsum/5mdp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mdp ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mdp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mdp OCA], [https://pdbe.org/5mdp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mdp RCSB], [https://www.ebi.ac.uk/pdbsum/5mdp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mdp ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/L0RVU0_VIBHA L0RVU0_VIBHA] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Achromobacter harveyi johnson and shunk 1936]] | + | [[Category: Large Structures]] |
| - | [[Category: Berg, B van den]]
| + | |
| - | [[Category: Zahn, M]]
| + | |
| - | [[Category: Outer membrane protein]]
| + | |
| - | [[Category: Porin channel]]
| + | |
| - | [[Category: Sugar binding protein]]
| + | |
| | [[Category: Vibrio harveyi]] | | [[Category: Vibrio harveyi]] |
| | + | [[Category: Zahn M]] |
| | + | [[Category: Van den Berg B]] |
| Structural highlights
Function
L0RVU0_VIBHA
Publication Abstract from PubMed
Chitin, an insoluble polymer of N-acetylglucosamine, is one of the most abundant biopolymers on Earth. By degrading chitin, chitinolytic bacteria such as Vibrio harveyi are critical for chitin recycling and maintenance of carbon and nitrogen cycles in the world's oceans. A decisive step in chitin degradation is the uptake of chito-oligosaccharides by an outer membrane protein channel named chitoporin (ChiP). Here, we report X-ray crystal structures of ChiP from V. harveyi in the presence and absence of chito-oligosaccharides. Structures without bound sugar reveal a trimeric assembly with an unprecedented closing of the transport pore by the N-terminus of a neighboring subunit. Substrate binding ejects the pore plug to open the transport channel. Together with molecular dynamics simulations, electrophysiology and in vitro transport assays our data provide an explanation for the exceptional affinity of ChiP for chito-oligosaccharides and point to an important role of the N-terminal gate in substrate transport.
Structural basis for chitin acquisition by marine Vibrio species.,Aunkham A, Zahn M, Kesireddy A, Pothula KR, Schulte A, Basle A, Kleinekathofer U, Suginta W, van den Berg B Nat Commun. 2018 Jan 15;9(1):220. doi: 10.1038/s41467-017-02523-y. PMID:29335469[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Aunkham A, Zahn M, Kesireddy A, Pothula KR, Schulte A, Basle A, Kleinekathofer U, Suginta W, van den Berg B. Structural basis for chitin acquisition by marine Vibrio species. Nat Commun. 2018 Jan 15;9(1):220. doi: 10.1038/s41467-017-02523-y. PMID:29335469 doi:http://dx.doi.org/10.1038/s41467-017-02523-y
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