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| | ==Extracellular domain of human CD83 - rhombohedral crystal form== | | ==Extracellular domain of human CD83 - rhombohedral crystal form== |
| - | <StructureSection load='5mj1' size='340' side='right' caption='[[5mj1]], [[Resolution|resolution]] 1.80Å' scene=''> | + | <StructureSection load='5mj1' size='340' side='right'caption='[[5mj1]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5mj1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MJ1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MJ1 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5mj1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MJ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MJ1 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5mix|5mix]], [[5mj0|5mj0]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CD83 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mj1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mj1 OCA], [https://pdbe.org/5mj1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mj1 RCSB], [https://www.ebi.ac.uk/pdbsum/5mj1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mj1 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mj1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mj1 OCA], [http://pdbe.org/5mj1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mj1 RCSB], [http://www.ebi.ac.uk/pdbsum/5mj1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mj1 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CD83_HUMAN CD83_HUMAN]] May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation. | + | [https://www.uniprot.org/uniprot/CD83_HUMAN CD83_HUMAN] May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Egerer-Sieber, C]] | + | [[Category: Large Structures]] |
| - | [[Category: Klingl, S]] | + | [[Category: Egerer-Sieber C]] |
| - | [[Category: Muller, Y A]] | + | [[Category: Klingl S]] |
| - | [[Category: Schmid, B]] | + | [[Category: Muller YA]] |
| - | [[Category: Weiler, S]] | + | [[Category: Schmid B]] |
| - | [[Category: Dendritic cell]]
| + | [[Category: Weiler S]] |
| - | [[Category: Immune system]]
| + | |
| - | [[Category: Immunoglobulin]]
| + | |
| - | [[Category: Receptor]]
| + | |
| Structural highlights
Function
CD83_HUMAN May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.
Publication Abstract from PubMed
CD83 is a type-I membrane protein and an efficient marker for identifying mature dendritic cells. Whereas membrane-bound full-length CD83 co-stimulates the immune system, a soluble variant (sCD83), consisting of the extracellular domain, only, displays strong immune-suppressive activities. Besides a prediction that sCD83 adopts a V-set Ig-like fold, however, little is known about the molecular architecture of CD83 and the mechanism by which CD83 exerts is function on dendritic cells and additional immune cells. Here, we report the crystal structure of human sCD83 up to a resolution of 1.7A solved in three different crystal forms. Interestingly, beta-strands C', C and D that are typical for V-set Ig-domains could not be traced in sCD83. Mass spectrometry analyses, limited proteolysis experiments and bioinformatics studies show that the corresponding segment displays enhanced main-chain accessibility, an extraordinary low sequence conservation and a predicted high disorder propensity. Chimeric proteins with amino acids swaps in this segment show unaltered immune-suppressive activities in a TNF-alpha assay when compared to wild-type sCD83. This strongly indicates that this segment does not participate in the biological activity of CD83. The crystal structure of CD83 shows the recurrent formation of dimers and trimers in the various crystal forms and reveals strong structural similarities between sCD83 and B7 family members as well as CD48, a SLAM family member. This suggests that CD83 exerts its immunological activity by mixed homotypic and heterotypic interactions as typically observed for proteins present in the immunological synapse.
Crystal structure of the extracellular domain of the human dendritic cell surface marker CD83.,Heilingloh CS, Klingl S, Egerer-Sieber C, Schmid B, Weiler S, Muhl-Zurbes P, Hofmann J, Stump JD, Sticht H, Kummer M, Steinkasserer A, Muller YA J Mol Biol. 2017 Mar 14. pii: S0022-2836(17)30113-4. doi:, 10.1016/j.jmb.2017.03.009. PMID:28315353[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Heilingloh CS, Klingl S, Egerer-Sieber C, Schmid B, Weiler S, Muhl-Zurbes P, Hofmann J, Stump JD, Sticht H, Kummer M, Steinkasserer A, Muller YA. Crystal structure of the extracellular domain of the human dendritic cell surface marker CD83. J Mol Biol. 2017 Mar 14. pii: S0022-2836(17)30113-4. doi:, 10.1016/j.jmb.2017.03.009. PMID:28315353 doi:http://dx.doi.org/10.1016/j.jmb.2017.03.009
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