3vyx
From Proteopedia
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==Structural insights into RISC assembly facilitated by dsRNA binding domains of human RNA helicase (DHX9)== | ==Structural insights into RISC assembly facilitated by dsRNA binding domains of human RNA helicase (DHX9)== | ||
| - | <StructureSection load='3vyx' size='340' side='right'caption='[[3vyx]]' scene=''> | + | <StructureSection load='3vyx' size='340' side='right'caption='[[3vyx]], [[Resolution|resolution]] 2.29Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VYX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VYX FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3vyx]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VYX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VYX FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vyx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vyx OCA], [https://pdbe.org/3vyx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vyx RCSB], [https://www.ebi.ac.uk/pdbsum/3vyx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vyx ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.29Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vyx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vyx OCA], [https://pdbe.org/3vyx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vyx RCSB], [https://www.ebi.ac.uk/pdbsum/3vyx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vyx ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/DHX9_HUMAN DHX9_HUMAN] Unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD-mediated complex that promotes MYC mRNA stability. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms. Positively regulates HIV-1 LTR-directed gene expression.<ref>PMID:19029303</ref> <ref>PMID:19229320</ref> <ref>PMID:22190748</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Intensive research interest has focused on small RNA-processing machinery and the RNA-induced silencing complex (RISC), key cellular machines in RNAi pathways. However, the structural mechanism regarding RISC assembly, the primary step linking small RNA processing and RNA-mediated gene silencing, is largely unknown. Human RNA helicase A (DHX9) was reported to function as an RISC-loading factor, and such function is mediated mainly by its dsRNA-binding domains (dsRBDs). Here, we report the crystal structures of human RNA helicase A (RHA) dsRBD1 and dsRBD2 domains in complex with dsRNAs, respectively. Structural analysis not only reveals higher siRNA duplex-binding affinity displayed by dsRBD1, but also identifies a crystallographic dsRBD1 pair of physiological significance in cooperatively recognizing dsRNAs. Structural observations are further validated by isothermal titration calorimetric (ITC) assay. Moreover, co-immunoprecipitation (co-IP) assay coupled with mutagenesis demonstrated that both dsRBDs are required for RISC association, and such association is mediated by dsRNA. Hence, our structural and functional efforts have revealed a potential working model for siRNA recognition by RHA tandem dsRBDs, and together they provide direct structural insights into RISC assembly facilitated by RHA. | ||
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| + | Structural insights into RISC assembly facilitated by dsRNA-binding domains of human RNA helicase A (DHX9).,Fu Q, Yuan YA Nucleic Acids Res. 2013 Jan 29. PMID:23361462<ref>PMID:23361462</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 3vyx" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[Helicase 3D structures|Helicase 3D structures]] | *[[Helicase 3D structures|Helicase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Fu Q]] | [[Category: Fu Q]] | ||
[[Category: Yuan YA]] | [[Category: Yuan YA]] | ||
Revision as of 12:42, 8 November 2023
Structural insights into RISC assembly facilitated by dsRNA binding domains of human RNA helicase (DHX9)
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