3wu0
From Proteopedia
(Difference between revisions)
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==Crystal structure of phosphorylated ETS-1 DNA binding and autoinhibitory domains (276-441)== | ==Crystal structure of phosphorylated ETS-1 DNA binding and autoinhibitory domains (276-441)== | ||
| - | <StructureSection load='3wu0' size='340' side='right'caption='[[3wu0]]' scene=''> | + | <StructureSection load='3wu0' size='340' side='right'caption='[[3wu0]], [[Resolution|resolution]] 2.60Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WU0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WU0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3wu0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WU0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WU0 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wu0 OCA], [https://pdbe.org/3wu0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wu0 RCSB], [https://www.ebi.ac.uk/pdbsum/3wu0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wu0 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wu0 OCA], [https://pdbe.org/3wu0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wu0 RCSB], [https://www.ebi.ac.uk/pdbsum/3wu0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wu0 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/ETS1_HUMAN ETS1_HUMAN] Transcription factor.<ref>PMID:10698492</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Cooperative assemblies of transcription factors (TFs) on target gene enhancers coordinate cell proliferation, fate specification, and differentiation through precise and complicated transcriptional mechanisms. Chemical modifications, such as phosphorylation, of TFs induced by cell signaling, further modulate the dynamic cooperativity of TFs. In this study, we found that various Ets1-containing TF-DNA complexes respond differently to calcium-induced phosphorylation of Ets1, which is known to inhibit Ets1-DNA binding. Crystallographic analysis of a complex comprising Ets1, Runx1, and CBFbeta at the TCRalpha enhancer, revealed that Ets1 acquires robust binding stability in the Runx1 and DNA-complexed state, via allosteric mechanisms. This allows phosphorylated Ets1 to be retained at the TCRalpha enhancer with Runx1, in contrast to other Ets1 target gene enhancers including mb-1 and stromelysin-1. This study provides a structure-based model for cell signaling-dependent regulation of target genes, mediated via chemical modification of TFs. | ||
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| + | A novel allosteric mechanism on protein-DNA interactions underlying the phosphorylation-dependent regulation of Ets1 target gene expressions.,Shiina M, Hamada K, Inoue-Bungo T, Shimamura M, Uchiyama A, Baba S, Sato K, Yamamoto M, Ogata K J Mol Biol. 2014 Jul 29. pii: S0022-2836(14)00368-4. doi:, 10.1016/j.jmb.2014.07.020. PMID:25083921<ref>PMID:25083921</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 3wu0" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Hamada K]] | [[Category: Hamada K]] | ||
[[Category: Ogata K]] | [[Category: Ogata K]] | ||
[[Category: Shiina M]] | [[Category: Shiina M]] | ||
Current revision
Crystal structure of phosphorylated ETS-1 DNA binding and autoinhibitory domains (276-441)
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