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2l3e

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Revision as of 17:28, 8 November 2023

Solution structure of P2a-J2a/b-P2b of human telomerase RNA

Structural highlights

2l3e is a 1 chain structure. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Telomerase is a unique ribonucleoprotein complex that catalyzes the addition of telomeric DNA repeats onto the 3' ends of linear chromosomes. All vertebrate telomerase RNAs contain a catalytically essential core domain that includes the template and a pseudoknot with extended helical subdomains. Within these helical regions is an asymmetric 5-nt internal bulge loop (J2a/b) flanked by helices (P2a and P2b) that is highly conserved in its location but not sequence. NMR structure determination reveals that J2a/b forms a defined S-shape and creates an approximately 90 degrees bend with a surprisingly low twist ( approximately 10 degrees ) between the flanking helices. A search of RNA structures revealed only one other example of a 5-nt bulge, from hepatitis C virus internal ribosome entry site, with a different sequence but the same structure. J2a/b is intrinsically flexible but the interhelical motions across the loop are remarkably restricted. Nucleotide substitutions in J2a/b that affect the bend angle, direction, and interhelical dynamics are correlated with telomerase activity. Based on the structures of P2ab (J2a/b and flanking helices), the conserved region of the pseudoknot (P2b/P3, previously determined) and the remaining helical segment (P2a.1-J2a.1 refined using residual dipolar couplings and the modeling program MC-Sym) we have calculated an NMR-based model of the full-length pseudoknot. The model and dynamics analysis show that J2a/b serves as a dominant structural and dynamical element in defining the overall topology of the core domain, and suggest that interhelical motions in P2ab facilitate nucleotide addition along the template and template translocation.

Structurally conserved five nucleotide bulge determines the overall topology of the core domain of human telomerase RNA.,Zhang Q, Kim NK, Peterson RD, Wang Z, Feigon J Proc Natl Acad Sci U S A. 2010 Nov 2;107(44):18761-8. Epub 2010 Oct 21. PMID:20966348^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zhang Q, Kim NK, Peterson RD, Wang Z, Feigon J. Structurally conserved five nucleotide bulge determines the overall topology of the core domain of human telomerase RNA. Proc Natl Acad Sci U S A. 2010 Nov 2;107(44):18761-8. Epub 2010 Oct 21. PMID:20966348 doi:10.1073/pnas.1013269107

1 Structural highlights
2 Publication Abstract from PubMed
3 See Also
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2l3e"

@@ Line 1: / Line 1: @@
 ==Solution structure of P2a-J2a/b-P2b of human telomerase RNA==
-<StructureSection load='2l3e' size='340' side='right'caption='[[2l3e]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2l3e' size='340' side='right'caption='[[2l3e]]' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[2l3e]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L3E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L3E FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l3e OCA], [https://pdbe.org/2l3e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l3e RCSB], [https://www.ebi.ac.uk/pdbsum/2l3e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l3e ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l3e OCA], [https://pdbe.org/2l3e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l3e RCSB], [https://www.ebi.ac.uk/pdbsum/2l3e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l3e ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 17: / Line 18: @@
 ==See Also==
-*[[Telomerase|Telomerase]]
+*[[Telomerase 3D structures|Telomerase 3D structures]]
 == References ==
 <references/>
@@ Line 23: / Line 24: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Feigon, J]]
+[[Category: Feigon J]]
-[[Category: Kim, N]]
+[[Category: Kim N]]
-[[Category: Peterson, R D]]
+[[Category: Peterson RD]]
-[[Category: Wang, Z]]
+[[Category: Wang Z]]
-[[Category: Zhang, Q]]
+[[Category: Zhang Q]]
-[[Category: Htr]]
-[[Category: Human telomerase rna]]
-[[Category: J2ab]]
-[[Category: P2a]]
-[[Category: P2b]]
-[[Category: Rna]]

2l3e

From Proteopedia

Revision as of 17:28, 8 November 2023

Solution structure of P2a-J2a/b-P2b of human telomerase RNA

Structural highlights

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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