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| | <StructureSection load='5mlt' size='340' side='right'caption='[[5mlt]], [[Resolution|resolution]] 1.61Å' scene=''> | | <StructureSection load='5mlt' size='340' side='right'caption='[[5mlt]], [[Resolution|resolution]] 1.61Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5mlt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strpn Strpn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MLT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5MLT FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5mlt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_TIGR4 Streptococcus pneumoniae TIGR4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MLT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MLT FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.61Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SP_0092 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=170187 STRPN])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mlt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mlt OCA], [https://pdbe.org/5mlt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mlt RCSB], [https://www.ebi.ac.uk/pdbsum/5mlt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mlt ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5mlt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mlt OCA], [http://pdbe.org/5mlt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mlt RCSB], [http://www.ebi.ac.uk/pdbsum/5mlt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mlt ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/SBP92_STRPN SBP92_STRPN] Probably part of an ABC transporter complex involved in carbohydrate transport.<ref>PMID:28045395</ref> <ref>PMID:28994793</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Strpn]] | + | [[Category: Streptococcus pneumoniae TIGR4]] |
| - | [[Category: Culurgioni, S]] | + | [[Category: Culurgioni S]] |
| - | [[Category: Tang, M]] | + | [[Category: Tang M]] |
| - | [[Category: Walsh, M A]] | + | [[Category: Walsh MA]] |
| - | [[Category: Substrate binding protein carbohydrate periplasmic binding protein type 2 superfamily]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Function
SBP92_STRPN Probably part of an ABC transporter complex involved in carbohydrate transport.[1] [2]
Publication Abstract from PubMed
Streptococcus pneumoniae is an opportunistic respiratory pathogen that remains a major cause of morbidity and mortality globally, with infants and the elderly at the highest risk. S. pneumoniae relies entirely on carbohydrates as a source of carbon and dedicates a third of all uptake systems to carbohydrate import. The structure of the carbohydrate-free substrate-binding protein SP0092 at 1.61 A resolution reveals it to belong to the newly proposed subclass G of substrate-binding proteins, with a ligand-binding pocket that is large enough to accommodate complex oligosaccharides. SP0092 is a dimer in solution and the crystal structure reveals a domain-swapped dimer with the monomer subunits in a closed conformation but in the absence of carbohydrate ligand. This closed conformation may be induced by dimer formation and could be used as a mechanism to regulate carbohydrate uptake.
Structural characterization of the Streptococcus pneumoniae carbohydrate substrate-binding protein SP0092.,Culurgioni S, Tang M, Walsh MA Acta Crystallogr F Struct Biol Commun. 2017 Jan 1;73(Pt 1):54-61. doi:, 10.1107/S2053230X16020252. Epub 2017 Jan 1. PMID:28045395[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Culurgioni S, Tang M, Walsh MA. Structural characterization of the Streptococcus pneumoniae carbohydrate substrate-binding protein SP0092. Acta Crystallogr F Struct Biol Commun. 2017 Jan 1;73(Pt 1):54-61. doi:, 10.1107/S2053230X16020252. Epub 2017 Jan 1. PMID:28045395 doi:http://dx.doi.org/10.1107/S2053230X16020252
- ↑ Culurgioni S, Tang M, Hall DR, Walsh MA. Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092. J Vis Exp. 2017 Oct 2;(128):56294. PMID:28994793 doi:10.3791/56294
- ↑ Culurgioni S, Tang M, Walsh MA. Structural characterization of the Streptococcus pneumoniae carbohydrate substrate-binding protein SP0092. Acta Crystallogr F Struct Biol Commun. 2017 Jan 1;73(Pt 1):54-61. doi:, 10.1107/S2053230X16020252. Epub 2017 Jan 1. PMID:28045395 doi:http://dx.doi.org/10.1107/S2053230X16020252
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