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| | ==Complex between the Leucine Zipper (LZ) and Centrosomin-motif 2 (CM2) domains of Drosophila melanogaster Centrosomin (Cnn) - L535E mutant form== | | ==Complex between the Leucine Zipper (LZ) and Centrosomin-motif 2 (CM2) domains of Drosophila melanogaster Centrosomin (Cnn) - L535E mutant form== |
| - | <StructureSection load='5mw9' size='340' side='right' caption='[[5mw9]], [[Resolution|resolution]] 2.20Å' scene=''> | + | <StructureSection load='5mw9' size='340' side='right'caption='[[5mw9]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5mw9]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MW9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MW9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5mw9]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MW9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MW9 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cnn, Arr, CG4832 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mw9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mw9 OCA], [http://pdbe.org/5mw9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mw9 RCSB], [http://www.ebi.ac.uk/pdbsum/5mw9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mw9 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mw9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mw9 OCA], [https://pdbe.org/5mw9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mw9 RCSB], [https://www.ebi.ac.uk/pdbsum/5mw9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mw9 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CNN_DROME CNN_DROME]] Core component of the centrosome throughout spermatogenesis. May participate in mitotic spindle assembly and the mechanics of morphogenesis through an interaction with microtubules, either directly or indirectly. Is a target of several homeotic genes.<ref>PMID:8653793</ref> <ref>PMID:9548723</ref> | + | [https://www.uniprot.org/uniprot/CNN_DROME CNN_DROME] Core component of the centrosome throughout spermatogenesis. May participate in mitotic spindle assembly and the mechanics of morphogenesis through an interaction with microtubules, either directly or indirectly. Is a target of several homeotic genes.<ref>PMID:8653793</ref> <ref>PMID:9548723</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Drome]] | + | [[Category: Drosophila melanogaster]] |
| - | [[Category: Feng, Z]] | + | [[Category: Large Structures]] |
| - | [[Category: Johnson, S]] | + | [[Category: Feng Z]] |
| - | [[Category: Lea, S M]] | + | [[Category: Johnson S]] |
| - | [[Category: Raff, J W]] | + | [[Category: Lea SM]] |
| - | [[Category: Cell cycle]] | + | [[Category: Raff JW]] |
| - | [[Category: Centriole]]
| + | |
| - | [[Category: Centrosome]]
| + | |
| - | [[Category: Coiled-coil]]
| + | |
| - | [[Category: Mitosis]]
| + | |
| Structural highlights
Function
CNN_DROME Core component of the centrosome throughout spermatogenesis. May participate in mitotic spindle assembly and the mechanics of morphogenesis through an interaction with microtubules, either directly or indirectly. Is a target of several homeotic genes.[1] [2]
Publication Abstract from PubMed
In flies, Centrosomin (Cnn) forms a phosphorylation-dependent scaffold that recruits proteins to the mitotic centrosome, but how Cnn assembles into a scaffold is unclear. We show that scaffold assembly requires conserved leucine zipper (LZ) and Cnn-motif 2 (CM2) domains that co-assemble into a 2:2 complex in vitro. We solve the crystal structure of the LZ:CM2 complex, revealing that both proteins form helical dimers that assemble into an unusual tetramer. A slightly longer version of the LZ can form micron-scale structures with CM2, whose assembly is stimulated by Plk1 phosphorylation in vitro. Mutating individual residues that perturb LZ:CM2 tetramer assembly perturbs the formation of these micron-scale assemblies in vitro and Cnn-scaffold assembly in vivo. Thus, Cnn molecules have an intrinsic ability to form large, LZ:CM2-interaction-dependent assemblies that are critical for mitotic centrosome assembly. These studies provide the first atomic insight into a molecular interaction required for mitotic centrosome assembly.
Structural Basis for Mitotic Centrosome Assembly in Flies.,Feng Z, Caballe A, Wainman A, Johnson S, Haensele AFM, Cottee MA, Conduit PT, Lea SM, Raff JW Cell. 2017 Jun 1;169(6):1078-1089.e13. doi: 10.1016/j.cell.2017.05.030. PMID:28575671[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Li K, Kaufman TC. The homeotic target gene centrosomin encodes an essential centrosomal component. Cell. 1996 May 17;85(4):585-96. PMID:8653793
- ↑ Li K, Xu EY, Cecil JK, Turner FR, Megraw TL, Kaufman TC. Drosophila centrosomin protein is required for male meiosis and assembly of the flagellar axoneme. J Cell Biol. 1998 Apr 20;141(2):455-67. PMID:9548723
- ↑ Feng Z, Caballe A, Wainman A, Johnson S, Haensele AFM, Cottee MA, Conduit PT, Lea SM, Raff JW. Structural Basis for Mitotic Centrosome Assembly in Flies. Cell. 2017 Jun 1;169(6):1078-1089.e13. doi: 10.1016/j.cell.2017.05.030. PMID:28575671 doi:http://dx.doi.org/10.1016/j.cell.2017.05.030
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