1o7k

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[[Image:1o7k.gif|left|200px]]
[[Image:1o7k.gif|left|200px]]
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{{Structure
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|PDB= 1o7k |SIZE=350|CAPTION= <scene name='initialview01'>1o7k</scene>, resolution 2.0&Aring;
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The line below this paragraph, containing "STRUCTURE_1o7k", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=API:Second+Anionic+Lipid+Binding+Site.+Chain+C'>API</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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{{STRUCTURE_1o7k| PDB=1o7k | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7k OCA], [http://www.ebi.ac.uk/pdbsum/1o7k PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1o7k RCSB]</span>
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'''HUMAN P47 PX DOMAIN COMPLEX WITH SULPHATES'''
'''HUMAN P47 PX DOMAIN COMPLEX WITH SULPHATES'''
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[[Category: Perisic, O.]]
[[Category: Perisic, O.]]
[[Category: Williams, R L.]]
[[Category: Williams, R L.]]
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[[Category: nadph oxidase]]
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[[Category: Nadph oxidase]]
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[[Category: p47]]
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[[Category: P47]]
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[[Category: phosphoinositide-binding]]
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[[Category: Phosphoinositide-binding]]
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[[Category: phospholipid-binding]]
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[[Category: Phospholipid-binding]]
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[[Category: px domain]]
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[[Category: Px domain]]
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[[Category: sh3 domain]]
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[[Category: Sh3 domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:29:06 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:40:58 2008''
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Revision as of 00:29, 3 May 2008

Image:1o7k.gif

Template:STRUCTURE 1o7k

HUMAN P47 PX DOMAIN COMPLEX WITH SULPHATES


Overview

p47(phox) is a key cytosolic subunit required for activation of phagocyte NADPH oxidase. The X-ray structure of the p47(phox) PX domain revealed two distinct basic pockets on the membrane-binding surface, each occupied by a sulfate. These two pockets have different specificities: one preferentially binds phosphatidylinositol 3,4-bisphosphate [PtdIns(3,4)P(2)] and is analogous to the phophatidylinositol 3-phosphate (PtdIns3P)-binding pocket of p40(phox), while the other binds anionic phospholipids such as phosphatidic acid (PtdOH) or phosphatidylserine. The preference of this second site for PtdOH may be related to previously observed activation of NADPH oxidase by PtdOH. Simultaneous occupancy of the two phospholipid-binding pockets radically increases membrane affinity. Strikingly, measurements for full-length p47(phox) show that membrane interaction by the PX domain is masked by an intramolecular association with the C-terminal SH3 domain (C-SH3). Either a site-specific mutation in C-SH3 (W263R) or a mimic of the phosphorylated form of p47(phox) [Ser(303, 304, 328, 359, 370)Glu] cause a transition from a closed to an open conformation that binds membranes with a greater affinity than the isolated PX domain.

About this Structure

1O7K is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Binding of the PX domain of p47(phox) to phosphatidylinositol 3,4-bisphosphate and phosphatidic acid is masked by an intramolecular interaction., Karathanassis D, Stahelin RV, Bravo J, Perisic O, Pacold CM, Cho W, Williams RL, EMBO J. 2002 Oct 1;21(19):5057-68. PMID:12356722 Page seeded by OCA on Sat May 3 03:29:06 2008

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OCA

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