1o7l

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[[Image:1o7l.jpg|left|200px]]
[[Image:1o7l.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1o7l |SIZE=350|CAPTION= <scene name='initialview01'>1o7l</scene>, resolution 2.75&Aring;
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The line below this paragraph, containing "STRUCTURE_1o7l", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=MO1:Ca+Binding+Site+For+Chain+D'>MO1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MOO:MOLYBDATE+ION'>MOO</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1o7l| PDB=1o7l | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o7l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7l OCA], [http://www.ebi.ac.uk/pdbsum/1o7l PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1o7l RCSB]</span>
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}}
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'''MOLYBDATE-ACTIVATED FORM OF MODE FROM ESCHERICHIA COLI'''
'''MOLYBDATE-ACTIVATED FORM OF MODE FROM ESCHERICHIA COLI'''
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[[Category: Hunter, W N.]]
[[Category: Hunter, W N.]]
[[Category: Schuttelkopf, A W.]]
[[Category: Schuttelkopf, A W.]]
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[[Category: dna binding]]
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[[Category: Dna binding]]
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[[Category: molybdate]]
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[[Category: Molybdate]]
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[[Category: mop]]
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[[Category: Mop]]
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[[Category: transcriptional regulator,activator]]
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[[Category: Transcriptional regulator,activator]]
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[[Category: winged helix-turn-helix]]
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[[Category: Winged helix-turn-helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:29:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:41:03 2008''
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Revision as of 00:29, 3 May 2008

Image:1o7l.jpg

Template:STRUCTURE 1o7l

MOLYBDATE-ACTIVATED FORM OF MODE FROM ESCHERICHIA COLI


Overview

ModE is a bacterial transcriptional regulator that orchestrates many aspects of molybdenum metabolism by binding to specific DNA sequences in a molybdate-dependent fashion. We present the crystal structure of Escherichia coli ModE in complex with molybdate, which was determined at 2.75A from a merohedrally twinned crystal (twin fraction approximately 0.30) with space group P4(3). We now have structures of ModE in both its "switched on" (ligand-bound) and "switched off" (apo) states. Comparison with the apo structure shows that ligand binding leads to extensive conformational changes not only in the molybdate-binding domain, but also in the DNA-binding domain. The most obvious difference is the loss of the pronounced asymmetry between the two chains of the ModE dimer, which had been a characteristic property of the apo structure. Another major change concerns the relative orientation of the two DNA-interacting winged helix-turn-helix motifs. Manual docking of an idealized DNA structure suggests that this conformational change should improve DNA binding of the activated molybdate-bound ModE.

About this Structure

1O7L is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of activated ModE reveals conformational changes involving both oxyanion and DNA-binding domains., Schuttelkopf AW, Boxer DH, Hunter WN, J Mol Biol. 2003 Feb 21;326(3):761-7. PMID:12581638 Page seeded by OCA on Sat May 3 03:29:08 2008

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