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| | <StructureSection load='5n1a' size='340' side='right'caption='[[5n1a]], [[Resolution|resolution]] 2.15Å' scene=''> | | <StructureSection load='5n1a' size='340' side='right'caption='[[5n1a]], [[Resolution|resolution]] 2.15Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5n1a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chatd Chatd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N1A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N1A FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5n1a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N1A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5N1A FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CTHT_0058380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=759272 CHATD])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n1a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n1a OCA], [http://pdbe.org/5n1a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n1a RCSB], [http://www.ebi.ac.uk/pdbsum/5n1a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n1a ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5n1a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n1a OCA], [https://pdbe.org/5n1a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5n1a RCSB], [https://www.ebi.ac.uk/pdbsum/5n1a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5n1a ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/G0SCT7_CHATD G0SCT7_CHATD] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Chatd]] | + | [[Category: Chaetomium thermophilum var. thermophilum DSM 1495]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Ahmed, Y L]] | + | [[Category: Ahmed YL]] |
| - | [[Category: Calvino, F R]] | + | [[Category: Calvino FR]] |
| - | [[Category: Sinning, I]] | + | [[Category: Sinning I]] |
| - | [[Category: Wild, K]] | + | [[Category: Wild K]] |
| - | [[Category: Ribosome]]
| + | |
| - | [[Category: Ribosome biogenesis]]
| + | |
| - | [[Category: Translation]]
| + | |
| Structural highlights
Function
G0SCT7_CHATD
Publication Abstract from PubMed
Eukaryotic ribosome biogenesis begins with the co-transcriptional assembly of the 90S pre-ribosome. The 'U three protein' (UTP) complexes and snoRNP particles arrange around the nascent pre-ribosomal RNA chaperoning its folding and further maturation. The earliest event in this hierarchical process is the binding of the UTP-A complex to the 5'-end of the pre-ribosomal RNA (5'-ETS). This oligomeric complex predominantly consists of beta-propeller and alpha-solenoidal proteins. Here we present the structure of the Utp4 subunit from the thermophilic fungus Chaetomium thermophilum at 2.15 A resolution and analyze its function by UV RNA-crosslinking (CRAC) and in context of a recent cryo-EM structure of the 90S pre-ribosome. Utp4 consists of two orthogonal and highly basic beta-propellers that perfectly fit the EM-data. The Utp4 structure highlights an unusual Velcro-closure of its C-terminal beta-propeller as relevant for protein integrity and potentially Utp8 recognition in the context of the pre-ribosome. We provide a first model of the 5'-ETS RNA from the internally hidden 5'-end up to the region that hybridizes to the 3'-hinge sequence of U3 snoRNA and validate a specific Utp4/5'-ETS interaction by CRAC analysis.
Structural basis for 5'-ETS recognition by Utp4 at the early stages of ribosome biogenesis.,Calvino FR, Kornprobst M, Schermann G, Birkle F, Wild K, Fischer T, Hurt E, Ahmed YL, Sinning I PLoS One. 2017 Jun 2;12(6):e0178752. doi: 10.1371/journal.pone.0178752., eCollection 2017. PMID:28575120[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Calvino FR, Kornprobst M, Schermann G, Birkle F, Wild K, Fischer T, Hurt E, Ahmed YL, Sinning I. Structural basis for 5'-ETS recognition by Utp4 at the early stages of ribosome biogenesis. PLoS One. 2017 Jun 2;12(6):e0178752. doi: 10.1371/journal.pone.0178752., eCollection 2017. PMID:28575120 doi:http://dx.doi.org/10.1371/journal.pone.0178752
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