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| | <SX load='5n5n' size='340' side='right' viewer='molstar' caption='[[5n5n]], [[Resolution|resolution]] 4.00Å' scene=''> | | <SX load='5n5n' size='340' side='right' viewer='molstar' caption='[[5n5n]], [[Resolution|resolution]] 4.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5n5n]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N5N OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5N5N FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5n5n]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N5N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5N5N FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TUBB, TUBB5, OK/SW-cl.56 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), TUBA1B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5n5n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n5n OCA], [http://pdbe.org/5n5n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n5n RCSB], [http://www.ebi.ac.uk/pdbsum/5n5n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n5n ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5n5n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n5n OCA], [https://pdbe.org/5n5n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5n5n RCSB], [https://www.ebi.ac.uk/pdbsum/5n5n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5n5n ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TBB5_HUMAN TBB5_HUMAN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[http://www.uniprot.org/uniprot/TBA1B_HUMAN TBA1B_HUMAN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. | + | [https://www.uniprot.org/uniprot/TBB5_HUMAN TBB5_HUMAN] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Atherton, J]] | + | [[Category: Atherton J]] |
| - | [[Category: Moores, C A]] | + | [[Category: Moores CA]] |
| - | [[Category: Roll-Mecak, A]] | + | [[Category: Roll-Mecak A]] |
| - | [[Category: Spector, J O]] | + | [[Category: Spector JO]] |
| - | [[Category: Vemu, A]] | + | [[Category: Vemu A]] |
| - | [[Category: Microtubules dynamics tubulin isoform]]
| + | |
| - | [[Category: Structural protein]]
| + | |
| Structural highlights
Function
TBB5_HUMAN Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Publication Abstract from PubMed
Microtubules polymerize and depolymerize stochastically, a behavior essential for cell division, motility and differentiation. While many studies advanced our understanding of how microtubule-associated proteins tune microtubule dynamics in trans, we have yet to understand how tubulin genetic diversity regulates microtubule functions. The majority of in vitro dynamics studies are performed with tubulin purified from brain tissue. This preparation is not representative of tubulin found in many cell types. Here we report the 4.2A cryo-EM structure and in vitro dynamics parameters of alpha1B/betaI+betaIVb microtubules assembled from tubulin purified from a human embryonic kidney cell line with isoform composition characteristic of fibroblasts and many immortalized cell lines. We find that these microtubules grow faster and transition to depolymerization less frequently compared to brain microtubules. Cryo-EM reveals that the dynamic ends of alpha1B/betaI+betaIVb microtubules are less tapered and that these tubulin heterodimers display lower curvatures. Interestingly, analysis of EB1 distributions at dynamic ends suggests no differences in GTP cap sizes. Lastly, we show that the addition of recombinant alpha1A/betaIII tubulin, a neuronal isotype overexpressed in many tumors, proportionally tunes the dynamics of alpha1B/betaI+betaIVb microtubules. Our study is an important step towards understanding how tubulin isoform composition tunes microtubule dynamics.
Tubulin isoform composition tunes microtubule dynamics.,Vemu A, Atherton J, Spector JO, Moores CA, Roll-Mecak A Mol Biol Cell. 2017 Oct 11. pii: mbc.E17-02-0124. doi: 10.1091/mbc.E17-02-0124. PMID:29021343[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Vemu A, Atherton J, Spector JO, Moores CA, Roll-Mecak A. Tubulin isoform composition tunes microtubule dynamics. Mol Biol Cell. 2017 Oct 11. pii: mbc.E17-02-0124. doi: 10.1091/mbc.E17-02-0124. PMID:29021343 doi:http://dx.doi.org/10.1091/mbc.E17-02-0124
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