8c0t
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==NRS 1.2: Fluorescent Sensors for Imaging Interstitial Calcium== | |
| + | <StructureSection load='8c0t' size='340' side='right'caption='[[8c0t]], [[Resolution|resolution]] 1.28Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8c0t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Branchiostoma_lanceolatum Branchiostoma lanceolatum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8C0T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8C0T FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.28Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8c0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8c0t OCA], [https://pdbe.org/8c0t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8c0t RCSB], [https://www.ebi.ac.uk/pdbsum/8c0t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8c0t ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Calcium in interstitial fluids is central to systemic physiology and a crucial ion pool for entry into cells through numerous plasma membrane channels. Its study has been limited by the scarcity of methods that allow monitoring in tight inter-cell spaces of living tissues. Here we present high performance ultra-low affinity genetically encoded calcium biosensors named GreenT-ECs. GreenT-ECs combine large fluorescence changes upon calcium binding and binding affinities (Kds) ranging from 0.8 mM to 2.9 mM, making them tuned to calcium concentrations in extracellular organismal fluids. We validated GreenT-ECs in rodent hippocampal neurons and transgenic zebrafish in vivo, where the sensors enabled monitoring homeostatic regulation of tissue interstitial calcium. GreenT-ECs may become useful for recording very large calcium transients and for imaging calcium homeostasis in inter-cell structures in live tissues and organisms. | ||
| - | + | Fluorescent sensors for imaging of interstitial calcium.,Valiente-Gabioud AA, Garteizgogeascoa Suner I, Idziak A, Fabritius A, Basquin J, Angibaud J, Nagerl UV, Singh SP, Griesbeck O Nat Commun. 2023 Oct 5;14(1):6220. doi: 10.1038/s41467-023-41928-w. PMID:37798285<ref>PMID:37798285</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Basquin | + | <div class="pdbe-citations 8c0t" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Branchiostoma lanceolatum]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Basquin J]] | ||
| + | [[Category: Griesbeck O]] | ||
| + | [[Category: Valiente-Gabioud A]] | ||
Revision as of 07:31, 15 November 2023
NRS 1.2: Fluorescent Sensors for Imaging Interstitial Calcium
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