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| | <StructureSection load='4b30' size='340' side='right'caption='[[4b30]], [[Resolution|resolution]] 2.10Å' scene=''> | | <StructureSection load='4b30' size='340' side='right'caption='[[4b30]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4b30]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Anthomedusae_sp._dc-2005 Anthomedusae sp. dc-2005]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B30 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B30 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4b30]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Anthomedusae_sp._DC-2005 Anthomedusae sp. DC-2005]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B30 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B30 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2wiq|2wiq]], [[2wis|2wis]]</div></td></tr> | + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b30 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b30 OCA], [https://pdbe.org/4b30 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b30 RCSB], [https://www.ebi.ac.uk/pdbsum/4b30 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b30 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b30 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b30 OCA], [https://pdbe.org/4b30 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b30 RCSB], [https://www.ebi.ac.uk/pdbsum/4b30 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b30 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q2TCH5_9CNID Q2TCH5_9CNID] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Anthomedusae sp. dc-2005]] | + | [[Category: Anthomedusae sp. DC-2005]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Carpentier, P]] | + | [[Category: Carpentier P]] |
| - | [[Category: Rosny, E de]] | + | [[Category: De Rosny E]] |
| - | [[Category: Fluorescent protein]]
| + | |
| - | [[Category: Photobleaching]]
| + | |
| - | [[Category: Photoconversion]]
| + | |
| - | [[Category: Ro]]
| + | |
| Structural highlights
4b30 is a 2 chain structure with sequence from Anthomedusae sp. DC-2005. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | X-ray diffraction, Resolution 2.1Å |
| Ligands: | , , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
Q2TCH5_9CNID
Publication Abstract from PubMed
KillerRed (KR) is a red fluorescent protein recognized as an efficient genetically encoded photosensitizer. KR generates reactive oxygen species via a complex process of photoreactions, ending up in photobleaching, the mechanism of which remains obscure. In order to clarify these mechanisms, we focus on a single mutant V44A (A44-KR) exhibiting the solely green component of KR. We report on the laser-induced structural transformations of A44-KR at cryogenic temperature, which we have investigated by combining UV-vis fluorescence/absorption spectroscopy with X-ray crystallography. Like the well-known GFP, A44-KR possesses a mixture of protonated (A) absorbing at 397 and deprotonated (B) absorbing at 515 nm chromophores, which are stressed by intense prolonged violet and blue laser sources. Both illuminations directly drive the B-chromophores toward a bleached trans isomerized form. A-type chromophores are sensitive only to violet illumination and are phototransformed either into a deprotonated green fluorescent form by decarboxylation of E218 or into a bleached form with a disordered p-hydroxybenzylidene. In crystallo spectroscopy at cryo-temperature allowed the identification and dissection of an exhaustive scheme of intermediates and end-products resulting from the phototransformation of A44-KR. This constitutes a framework for understanding the photochemistry of the photosensitizer KillerRed.
GFP-like phototransformation mechanisms in the cytotoxic fluorescent protein KillerRed unraveled by structural and spectroscopic investigations.,de Rosny E, Carpentier P J Am Chem Soc. 2012 Oct 31;134(43):18015-21. doi: 10.1021/ja3073337. Epub 2012, Oct 19. PMID:23025285[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ de Rosny E, Carpentier P. GFP-like phototransformation mechanisms in the cytotoxic fluorescent protein KillerRed unraveled by structural and spectroscopic investigations. J Am Chem Soc. 2012 Oct 31;134(43):18015-21. doi: 10.1021/ja3073337. Epub 2012, Oct 19. PMID:23025285 doi:http://dx.doi.org/10.1021/ja3073337
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