5ks8

From Proteopedia

(Difference between revisions)

Revision as of 09:48, 15 November 2023

Crystal structure of two-subunit pyruvate carboxylase from Methylobacillus flagellatus

Structural highlights

5ks8 is a 6 chain structure with sequence from Methylobacillus flagellatus KT. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.01Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q1H158_METFK

Publication Abstract from PubMed

Pyruvate carboxylase (PC) has important roles in metabolism and is crucial for virulence for some pathogenic bacteria. PC contains biotin carboxylase (BC), carboxyltransferase (CT) and biotin carboxyl carrier protein (BCCP) components. It is a single-chain enzyme in eukaryotes and most bacteria, and functions as a 500 kD homo-tetramer. In contrast, PC is a two-subunit enzyme in a collection of Gram-negative bacteria, with the alpha subunit containing the BC and the beta subunit the CT and BCCP domains, and it is believed that the holoenzyme has alpha4beta4 stoichiometry. We report here the crystal structures of a two-subunit PC from Methylobacillus flagellatus. Surprisingly, our structures reveal an alpha2beta4 stoichiometry, and the overall architecture of the holoenzyme is strikingly different from that of the homo-tetrameric PCs. Biochemical and mutagenesis studies confirm the stoichiometry and other structural observations. Our functional studies in Pseudomonas aeruginosa show that its two-subunit PC is important for colony morphogenesis.

A distinct holoenzyme organization for two-subunit pyruvate carboxylase.,Choi PH, Jo J, Lin YC, Lin MH, Chou CY, Dietrich LE, Tong L Nat Commun. 2016 Oct 6;7:12713. doi: 10.1038/ncomms12713. PMID:27708276^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Choi PH, Jo J, Lin YC, Lin MH, Chou CY, Dietrich LE, Tong L. A distinct holoenzyme organization for two-subunit pyruvate carboxylase. Nat Commun. 2016 Oct 6;7:12713. doi: 10.1038/ncomms12713. PMID:27708276 doi:http://dx.doi.org/10.1038/ncomms12713

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5ks8"

Categories: Large Structures | Methylobacillus flagellatus KT | Choi PH | Tong L

@@ Line 3: / Line 3: @@
 <StructureSection load='5ks8' size='340' side='right'caption='[[5ks8]], [[Resolution|resolution]] 3.01&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5ks8]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Metfk Metfk]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KS8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KS8 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5ks8]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylobacillus_flagellatus_KT Methylobacillus flagellatus KT]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KS8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KS8 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTI:5-(HEXAHYDRO-2-OXO-1H-THIENO[3,4-D]IMIDAZOL-6-YL)PENTANAL'>BTI</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PYR:PYRUVIC+ACID'>PYR</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.01&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Mfla_1511 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=265072 METFK]), Mfla_1512 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=265072 METFK])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTI:5-(HEXAHYDRO-2-OXO-1H-THIENO[3,4-D]IMIDAZOL-6-YL)PENTANAL'>BTI</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PYR:PYRUVIC+ACID'>PYR</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ks8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ks8 OCA], [https://pdbe.org/5ks8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ks8 RCSB], [https://www.ebi.ac.uk/pdbsum/5ks8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ks8 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q1H158_METFK Q1H158_METFK]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 17: / Line 19: @@
 </div>
 <div class="pdbe-citations 5ks8" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Pyruvate carboxylase 3D structures|Pyruvate carboxylase 3D structures]]
 == References ==
 <references/>
@@ Line 22: / Line 27: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Metfk]]
+[[Category: Methylobacillus flagellatus KT]]
-[[Category: Choi, P H]]
+[[Category: Choi PH]]
-[[Category: Tong, L]]
+[[Category: Tong L]]
-[[Category: Biotin]]
-[[Category: Ligase]]
-[[Category: Pyruvate]]
-[[Category: Tim barrel]]

5ks8

From Proteopedia

Revision as of 09:48, 15 November 2023

Crystal structure of two-subunit pyruvate carboxylase from Methylobacillus flagellatus

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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