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| | <StructureSection load='6po6' size='340' side='right'caption='[[6po6]], [[Resolution|resolution]] 1.00Å' scene=''> | | <StructureSection load='6po6' size='340' side='right'caption='[[6po6]], [[Resolution|resolution]] 1.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6po6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_syringae Pseudomonas syringae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PO6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PO6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6po6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_syringae Pseudomonas syringae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PO6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6PO6 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OV7:'>OV7</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron crystallography, [[Resolution|Resolution]] 1Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6po6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6po6 OCA], [http://pdbe.org/6po6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6po6 RCSB], [http://www.ebi.ac.uk/pdbsum/6po6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6po6 ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OV7:(2R)-amino[(carboxymethyl)sulfanyl]acetic+acid'>OV7</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6po6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6po6 OCA], [https://pdbe.org/6po6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6po6 RCSB], [https://www.ebi.ac.uk/pdbsum/6po6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6po6 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| | [[Category: Pseudomonas syringae]] | | [[Category: Pseudomonas syringae]] |
| - | [[Category: Donk, W A.van der]]
| + | [[Category: Funk MA]] |
| - | [[Category: Funk, M A]] | + | [[Category: Gonen T]] |
| - | [[Category: Gonen, T]] | + | [[Category: Halaby S]] |
| - | [[Category: Halaby, S]] | + | [[Category: Ting CP]] |
| - | [[Category: Ting, C P]] | + | [[Category: Van der Donk WA]] |
| - | [[Category: Microcrystal electron diffraction]] | + | |
| - | [[Category: Microed]]
| + | |
| - | [[Category: Ribosomal synthesized small peptide]]
| + | |
| - | [[Category: Unknown function]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Genome sequencing of environmental bacteria allows identification of biosynthetic gene clusters encoding unusual combinations of enzymes that produce unknown natural products. We identified a pathway in which a ribosomally synthesized small peptide serves as a scaffold for nonribosomal peptide extension and chemical modification. Amino acids are transferred to the carboxyl terminus of the peptide through adenosine triphosphate and amino acyl-tRNA-dependent chemistry that is independent of the ribosome. Oxidative rearrangement, carboxymethylation, and proteolysis of a terminal cysteine yields an amino acid-derived small molecule. Microcrystal electron diffraction demonstrates that the resulting product is isosteric to glutamate. We show that a similar peptide extension is used during the biosynthesis of the ammosamides, which are cytotoxic pyrroloquinoline alkaloids. These results suggest an alternative paradigm for biosynthesis of amino acid-derived natural products.
Use of a scaffold peptide in the biosynthesis of amino acid-derived natural products.,Ting CP, Funk MA, Halaby SL, Zhang Z, Gonen T, van der Donk WA Science. 2019 Jul 19;365(6450):280-284. doi: 10.1126/science.aau6232. PMID:31320540[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ting CP, Funk MA, Halaby SL, Zhang Z, Gonen T, van der Donk WA. Use of a scaffold peptide in the biosynthesis of amino acid-derived natural products. Science. 2019 Jul 19;365(6450):280-284. doi: 10.1126/science.aau6232. PMID:31320540 doi:http://dx.doi.org/10.1126/science.aau6232
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