|
|
| Line 3: |
Line 3: |
| | <StructureSection load='6rxh' size='340' side='right'caption='[[6rxh]], [[Resolution|resolution]] 2.00Å' scene=''> | | <StructureSection load='6rxh' size='340' side='right'caption='[[6rxh]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6rxh]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RXH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6RXH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6rxh]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RXH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RXH FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PYR:PYRUVIC+ACID'>PYR</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PYR:PYRUVIC+ACID'>PYR</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_1-decarboxylase Aspartate 1-decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.11 4.1.1.11] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rxh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rxh OCA], [https://pdbe.org/6rxh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rxh RCSB], [https://www.ebi.ac.uk/pdbsum/6rxh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rxh ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6rxh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rxh OCA], [http://pdbe.org/6rxh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rxh RCSB], [http://www.ebi.ac.uk/pdbsum/6rxh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rxh ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/A0A403CTL2_ECOLX A0A403CTL2_ECOLX]] Catalyzes the pyruvoyl-dependent decarboxylation of aspartate to produce beta-alanine.[HAMAP-Rule:MF_00446][SAAS:SAAS00097795] [[http://www.uniprot.org/uniprot/A0A3U0WEI2_ECOLX A0A3U0WEI2_ECOLX]] Catalyzes the pyruvoyl-dependent decarboxylation of aspartate to produce beta-alanine.[HAMAP-Rule:MF_00446][SAAS:SAAS00097795] | + | [https://www.uniprot.org/uniprot/PAND_ECOLI PAND_ECOLI] Catalyzes the pyruvoyl-dependent decarboxylation of aspartate to produce beta-alanine.<ref>PMID:6767707</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 20: |
Line 19: |
| | </div> | | </div> |
| | <div class="pdbe-citations 6rxh" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 6rxh" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Aspartate decarboxylase 3D structures|Aspartate decarboxylase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Aspartate 1-decarboxylase]] | + | [[Category: Escherichia coli]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Monteiro, D C.F]] | + | [[Category: Monteiro DCF]] |
| - | [[Category: Pearson, A R]] | + | [[Category: Pearson AR]] |
| - | [[Category: Stetten, D von]]
| + | [[Category: Trebbin M]] |
| - | [[Category: Trebbin, M]] | + | [[Category: Von Stetten D]] |
| - | [[Category: Lyase]] | + | |
| Structural highlights
Function
PAND_ECOLI Catalyzes the pyruvoyl-dependent decarboxylation of aspartate to produce beta-alanine.[1]
Publication Abstract from PubMed
Serial crystallography has enabled the study of complex biological questions through the determination of biomolecular structures at room temperature using low X-ray doses. Furthermore, it has enabled the study of protein dynamics by the capture of atomically resolved and time-resolved molecular movies. However, the study of many biologically relevant targets is still severely hindered by high sample consumption and lengthy data-collection times. By combining serial synchrotron crystallography (SSX) with 3D printing, a new experimental platform has been created that tackles these challenges. An affordable 3D-printed, X-ray-compatible microfluidic device (3D-MiXD) is reported that allows data to be collected from protein microcrystals in a 3D flow with very high hit and indexing rates, while keeping the sample consumption low. The miniaturized 3D-MiXD can be rapidly installed into virtually any synchrotron beamline with only minimal adjustments. This efficient collection scheme in combination with its mixing geometry paves the way for recording molecular movies at synchrotrons by mixing-triggered millisecond time-resolved SSX.
3D-MiXD: 3D-printed X-ray-compatible microfluidic devices for rapid, low-consumption serial synchrotron crystallography data collection in flow.,Monteiro DCF, von Stetten D, Stohrer C, Sans M, Pearson AR, Santoni G, van der Linden P, Trebbin M IUCrJ. 2020 Jan 16;7(Pt 2):207-219. doi: 10.1107/S2052252519016865. eCollection, 2020 Mar 1. PMID:32148849[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Cronan JE Jr. Beta-alanine synthesis in Escherichia coli. J Bacteriol. 1980 Mar;141(3):1291-7. PMID:6767707
- ↑ Monteiro DCF, von Stetten D, Stohrer C, Sans M, Pearson AR, Santoni G, van der Linden P, Trebbin M. 3D-MiXD: 3D-printed X-ray-compatible microfluidic devices for rapid, low-consumption serial synchrotron crystallography data collection in flow. IUCrJ. 2020 Jan 16;7(Pt 2):207-219. doi: 10.1107/S2052252519016865. eCollection, 2020 Mar 1. PMID:32148849 doi:http://dx.doi.org/10.1107/S2052252519016865
|
