7f21
From Proteopedia
(Difference between revisions)
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==L-lactate oxidase with D-lactate== | ==L-lactate oxidase with D-lactate== | ||
| - | <StructureSection load='7f21' size='340' side='right'caption='[[7f21]]' scene=''> | + | <StructureSection load='7f21' size='340' side='right'caption='[[7f21]], [[Resolution|resolution]] 1.38Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F21 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F21 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7f21]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aerococcus_viridans Aerococcus viridans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F21 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F21 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f21 OCA], [https://pdbe.org/7f21 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f21 RCSB], [https://www.ebi.ac.uk/pdbsum/7f21 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f21 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.38Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=LAC:LACTIC+ACID'>LAC</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f21 OCA], [https://pdbe.org/7f21 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f21 RCSB], [https://www.ebi.ac.uk/pdbsum/7f21 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f21 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/LOX_AERVM LOX_AERVM] Catalyzes the oxidation of (S)-lactate (L-lactate) to pyruvate, with a reduction of O2 to H2O2 (Ref.1, PubMed:27302031, PubMed:25423902, PubMed:2818595, PubMed:8589073, PubMed:26260739). Cannot oxidize D-lactate, glycolate, and D,L-2-hydroxybutanoate (PubMed:2818595). May be involved in the utilization of L-lactate as an energy source for growth (By similarity).[UniProtKB:O33655]<ref>PMID:25423902</ref> <ref>PMID:26260739</ref> <ref>PMID:27302031</ref> <ref>PMID:2818595</ref> <ref>PMID:8589073</ref> [UniProtKB:O33655] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The crystal structure of l-lactate oxidase in complex with l-lactate was solved at a 1.33 A resolution. The electron density of the bound l-lactate was clearly shown and comparisons of the free form and substrate bound complexes demonstrated that l-lactate was bound to the FMN and an additional active site within the enzyme complex. l-lactate interacted with the related side chains, which play an important role in enzymatic catalysis and especially the coupled movement of H265 and D174, which may be essential to activity. These observations not only reveal the enzymatic mechanism for l-lactate binding but also demonstrate the dynamic motion of these enzyme structures in response to substrate binding and enzymatic reaction progression. | ||
| + | |||
| + | Dynamic interactions in the l-lactate oxidase active site facilitate substrate binding at pH4.5.,Furubayashi N, Inaka K, Kamo M, Umena Y, Matsuoka T, Morimoto Y Biochem Biophys Res Commun. 2021 Sep 3;568:131-135. doi:, 10.1016/j.bbrc.2021.06.078. Epub 2021 Jun 30. PMID:34214876<ref>PMID:34214876</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7f21" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Aerococcus viridans]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Inaka K]] | [[Category: Inaka K]] | ||
[[Category: Morimoto Y]] | [[Category: Morimoto Y]] | ||
Current revision
L-lactate oxidase with D-lactate
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