1oa7
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1oa7.jpg|left|200px]] | [[Image:1oa7.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1oa7", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | | | + | --> |
| - | | | + | {{STRUCTURE_1oa7| PDB=1oa7 | SCENE= }} |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''STRUCTURE OF MELANOCARPUS ALBOMYCES ENDOGLUCANASE IN COMPLEX WITH CELLOBIOSE''' | '''STRUCTURE OF MELANOCARPUS ALBOMYCES ENDOGLUCANASE IN COMPLEX WITH CELLOBIOSE''' | ||
| Line 28: | Line 25: | ||
[[Category: Hirvonen, M.]] | [[Category: Hirvonen, M.]] | ||
[[Category: Papageorgiou, A C.]] | [[Category: Papageorgiou, A C.]] | ||
| - | [[Category: | + | [[Category: Cellulase]] |
| - | [[Category: | + | [[Category: Cellulose degradation]] |
| - | [[Category: | + | [[Category: Glycoside hydrolase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:35:13 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 00:35, 3 May 2008
STRUCTURE OF MELANOCARPUS ALBOMYCES ENDOGLUCANASE IN COMPLEX WITH CELLOBIOSE
Overview
Cellulose, a polysaccharide of beta-1,4-linked D-glucosyl units, is the major component of plant cell walls and one of the most abundant biopolymers in nature. Cellulases (cellobiohydrolases and endoglucanases) are enzymes that catalyse the hydrolysis of cellulose to smaller oligosaccharides, a process of paramount importance in biotechnology. The thermophilic fungus Melanocarpus albomyces produces a 20 kDa endoglucanase known as 20K-cellulase that has been found particularly useful in the textile industry. The crystal structures of free 20K-cellulase and its complex with cellobiose have been determined at 2.0 A resolution. The enzyme, classified into the glycoside hydrolase family 45, exhibits the characteristic six-stranded beta-barrel found before in Humicola insolens endoglucanase V structure. However, the active site in the 20K-cellulase shows a closing of approximately 2.5-3.5A while a mobile loop identified previously in Humicola insolens endoglucanase V and implicated in the catalytic mechanism is well-defined in 20K-cellulase. In addition, the crystal structure of the cellobiose complex shows a shift in the cellobiose position at the substrate-binding cleft. It is therefore proposed that these alterations may reflect differences in the binding mechanism and catalytic action of the enzyme.
About this Structure
1OA7 is a Single protein structure of sequence from Melanocarpus albomyces. Full crystallographic information is available from OCA.
Reference
Crystal structure of a family 45 endoglucanase from Melanocarpus albomyces: mechanistic implications based on the free and cellobiose-bound forms., Hirvonen M, Papageorgiou AC, J Mol Biol. 2003 Jun 6;329(3):403-10. PMID:12767825 Page seeded by OCA on Sat May 3 03:35:13 2008
