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| | <SX load='5n9y' size='340' side='right' viewer='molstar' caption='[[5n9y]], [[Resolution|resolution]] 4.20Å' scene=''> | | <SX load='5n9y' size='340' side='right' viewer='molstar' caption='[[5n9y]], [[Resolution|resolution]] 4.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5n9y]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N9Y OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5N9Y FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5n9y]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N9Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5N9Y FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">zntB, ydaN, b1342, JW1336 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.2Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5n9y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n9y OCA], [http://pdbe.org/5n9y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n9y RCSB], [http://www.ebi.ac.uk/pdbsum/5n9y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n9y ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5n9y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n9y OCA], [https://pdbe.org/5n9y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5n9y RCSB], [https://www.ebi.ac.uk/pdbsum/5n9y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5n9y ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ZNTB_ECOLI ZNTB_ECOLI]] Mediates efflux of zinc ions.[HAMAP-Rule:MF_01565] | + | [https://www.uniprot.org/uniprot/ZNTB_ECOLI ZNTB_ECOLI] Mediates efflux of zinc ions.[HAMAP-Rule:MF_01565] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| - | [[Category: Ecoli]] | + | [[Category: Escherichia coli K-12]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Cornelius, G]] | + | [[Category: Cornelius G]] |
| - | [[Category: Guskov, A]] | + | [[Category: Guskov A]] |
| - | [[Category: Scheres, S H.W]] | + | [[Category: Scheres SHW]] |
| - | [[Category: Slotboom, D J]] | + | [[Category: Slotboom DJ]] |
| - | [[Category: Stetsenko, A]] | + | [[Category: Stetsenko A]] |
| - | [[Category: Cora]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Zinc transport]]
| + | |
| - | [[Category: Zntb]]
| + | |
| Structural highlights
Function
ZNTB_ECOLI Mediates efflux of zinc ions.[HAMAP-Rule:MF_01565]
Publication Abstract from PubMed
Zinc is an essential microelement to sustain all forms of life. However, excess of zinc is toxic, therefore dedicated import, export and storage proteins for tight regulation of the zinc concentration have evolved. In Enterobacteriaceae, several membrane transporters are involved in zinc homeostasis and linked to virulence. ZntB has been proposed to play a role in the export of zinc, but the transport mechanism of ZntB is poorly understood and based only on experimental characterization of its distant homologue CorA magnesium channel. Here, we report the cryo-electron microscopy structure of full-length ZntB from Escherichia coli together with the results of isothermal titration calorimetry, and radio-ligand uptake and fluorescent transport assays on ZntB reconstituted into liposomes. Our results show that ZntB mediates Zn2+ uptake, stimulated by a pH gradient across the membrane, using a transport mechanism that does not resemble the one proposed for homologous CorA channels.
The structural basis of proton driven zinc transport by ZntB.,Gati C, Stetsenko A, Slotboom DJ, Scheres SHW, Guskov A Nat Commun. 2017 Nov 3;8(1):1313. doi: 10.1038/s41467-017-01483-7. PMID:29101379[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gati C, Stetsenko A, Slotboom DJ, Scheres SHW, Guskov A. The structural basis of proton driven zinc transport by ZntB. Nat Commun. 2017 Nov 3;8(1):1313. doi: 10.1038/s41467-017-01483-7. PMID:29101379 doi:http://dx.doi.org/10.1038/s41467-017-01483-7
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