5ncc

From Proteopedia

(Difference between revisions)

Current revision

Structure of Fatty acid Photodecarboxylase in complex with FAD and palmitic acid

Structural highlights

5ncc is a 6 chain structure with sequence from Chlorella variabilis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.12Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

FAP_CHLVA Catalyzes the decarboxylation of free fatty acids to n-alkanes or n-alkenes in response to blue light (PubMed:28860382, PubMed:30106504, PubMed:30673222). Substrate preference is toward fatty acids with C16 or C17 chains (PubMed:28860382, PubMed:30106504, PubMed:30673222). Saturated fatty acids are converted to alkanes, not alkenes (PubMed:28860382). The decarboxylation is initiated through electron abstraction from the fatty acid by the photo-excited FAD (PubMed:28860382).^[1] ^[2] ^[3]

Publication Abstract from PubMed

Although many organisms capture or respond to sunlight, few enzymes are known to be driven by light. Among these are DNA photolyases and the photosynthetic reaction centers. Here, we show that the microalga Chlorella variabilis NC64A harbors a photoenzyme that acts in lipid metabolism. This enzyme belongs to an algae-specific clade of the glucose-methanol-choline oxidoreductase family and catalyzes the decarboxylation of free fatty acids to n-alkanes or -alkenes in response to blue light. Crystal structure of the protein reveals a fatty acid-binding site in a hydrophobic tunnel leading to the light-capturing flavin adenine dinucleotide (FAD) cofactor. The decarboxylation is initiated through electron abstraction from the fatty acid by the photoexcited FAD with a quantum yield >80%. This photoenzyme, which we name fatty acid photodecarboxylase, may be useful in light-driven, bio-based production of hydrocarbons.

An algal photoenzyme converts fatty acids to hydrocarbons.,Sorigue D, Legeret B, Cuine S, Blangy S, Moulin S, Billon E, Richaud P, Brugiere S, Coute Y, Nurizzo D, Muller P, Brettel K, Pignol D, Arnoux P, Li-Beisson Y, Peltier G, Beisson F Science. 2017 Sep 1;357(6354):903-907. doi: 10.1126/science.aan6349. PMID:28860382^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sorigue D, Legeret B, Cuine S, Blangy S, Moulin S, Billon E, Richaud P, Brugiere S, Coute Y, Nurizzo D, Muller P, Brettel K, Pignol D, Arnoux P, Li-Beisson Y, Peltier G, Beisson F. An algal photoenzyme converts fatty acids to hydrocarbons. Science. 2017 Sep 1;357(6354):903-907. doi: 10.1126/science.aan6349. PMID:28860382 doi:http://dx.doi.org/10.1126/science.aan6349
↑ Huijbers MME, Zhang W, Tonin F, Hollmann F. Light-Driven Enzymatic Decarboxylation of Fatty Acids. Angew Chem Int Ed Engl. 2018 Oct 8;57(41):13648-13651. doi:, 10.1002/anie.201807119. Epub 2018 Sep 12. PMID:30106504 doi:http://dx.doi.org/10.1002/anie.201807119
↑ Zhang W, Ma M, Huijbers MME, Filonenko GA, Pidko EA, van Schie M, de Boer S, Burek BO, Bloh JZ, van Berkel WJH, Smith WA, Hollmann F. Hydrocarbon Synthesis via Photoenzymatic Decarboxylation of Carboxylic Acids. J Am Chem Soc. 2019 Feb 20;141(7):3116-3120. doi: 10.1021/jacs.8b12282. Epub 2019, Feb 6. PMID:30673222 doi:http://dx.doi.org/10.1021/jacs.8b12282
↑ Sorigue D, Legeret B, Cuine S, Blangy S, Moulin S, Billon E, Richaud P, Brugiere S, Coute Y, Nurizzo D, Muller P, Brettel K, Pignol D, Arnoux P, Li-Beisson Y, Peltier G, Beisson F. An algal photoenzyme converts fatty acids to hydrocarbons. Science. 2017 Sep 1;357(6354):903-907. doi: 10.1126/science.aan6349. PMID:28860382 doi:http://dx.doi.org/10.1126/science.aan6349

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5ncc"

@@ Line 3: / Line 3: @@
 <StructureSection load='5ncc' size='340' side='right'caption='[[5ncc]], [[Resolution|resolution]] 3.12&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5ncc]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlva Chlva]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NCC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NCC FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5ncc]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlorella_variabilis Chlorella variabilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NCC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NCC FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.12&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ncc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ncc OCA], [http://pdbe.org/5ncc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ncc RCSB], [http://www.ebi.ac.uk/pdbsum/5ncc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ncc ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ncc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ncc OCA], [https://pdbe.org/5ncc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ncc RCSB], [https://www.ebi.ac.uk/pdbsum/5ncc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ncc ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/FAP_CHLVA FAP_CHLVA] Catalyzes the decarboxylation of free fatty acids to n-alkanes or n-alkenes in response to blue light (PubMed:28860382, PubMed:30106504, PubMed:30673222). Substrate preference is toward fatty acids with C16 or C17 chains (PubMed:28860382, PubMed:30106504, PubMed:30673222). Saturated fatty acids are converted to alkanes, not alkenes (PubMed:28860382). The decarboxylation is initiated through electron abstraction from the fatty acid by the photo-excited FAD (PubMed:28860382).<ref>PMID:28860382</ref> <ref>PMID:30106504</ref> <ref>PMID:30673222</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Chlva]]
+[[Category: Chlorella variabilis]]
 [[Category: Large Structures]]
-[[Category: Arnoux, P]]
+[[Category: Arnoux P]]
-[[Category: Beisson, F]]
+[[Category: Beisson F]]
-[[Category: Pignol, D]]
+[[Category: Pignol D]]
-[[Category: Sorigue, D]]
+[[Category: Sorigue D]]
-[[Category: Oxidoreductase]]

5ncc

From Proteopedia

Current revision

Structure of Fatty acid Photodecarboxylase in complex with FAD and palmitic acid

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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