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| | ==Crystal structure of H. polymorpha ubiquitin conjugating enzyme Pex4p in complex with soluble domain of Pex22p== | | ==Crystal structure of H. polymorpha ubiquitin conjugating enzyme Pex4p in complex with soluble domain of Pex22p== |
| - | <StructureSection load='5nkz' size='340' side='right' caption='[[5nkz]], [[Resolution|resolution]] 2.85Å' scene=''> | + | <StructureSection load='5nkz' size='340' side='right'caption='[[5nkz]], [[Resolution|resolution]] 2.85Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5nkz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Cbs_7073 Cbs 7073]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NKZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NKZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5nkz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Ogataea_angusta Ogataea angusta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NKZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NKZ FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5nl8|5nl8]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PEX4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=870730 CBS 7073]), PEX22 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=870730 CBS 7073])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nkz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nkz OCA], [https://pdbe.org/5nkz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nkz RCSB], [https://www.ebi.ac.uk/pdbsum/5nkz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nkz ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/E2_ubiquitin-conjugating_enzyme E2 ubiquitin-conjugating enzyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.2.23 2.3.2.23] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nkz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nkz OCA], [http://pdbe.org/5nkz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nkz RCSB], [http://www.ebi.ac.uk/pdbsum/5nkz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nkz ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/UBCX_PICAN UBCX_PICAN]] Catalyzes the covalent attachment of ubiquitin to other proteins. Essential for peroxisome biogenesis. Required for UBC4-independent ubiquitination of PEX5.[PROSITE-ProRule:PRU00388] | + | [https://www.uniprot.org/uniprot/UBCX_PICAN UBCX_PICAN] Catalyzes the covalent attachment of ubiquitin to other proteins. Essential for peroxisome biogenesis. Required for UBC4-independent ubiquitination of PEX5.[PROSITE-ProRule:PRU00388] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Cbs 7073]] | + | [[Category: Large Structures]] |
| - | [[Category: E2 ubiquitin-conjugating enzyme]] | + | [[Category: Ogataea angusta]] |
| - | [[Category: Ali, A]] | + | [[Category: Ali A]] |
| - | [[Category: Danda, N]] | + | [[Category: Danda N]] |
| - | [[Category: Groves, M R]] | + | [[Category: Groves MR]] |
| - | [[Category: Lunev, S]] | + | [[Category: Lunev S]] |
| - | [[Category: Williams, C]] | + | [[Category: Williams C]] |
| - | [[Category: Crystallization]]
| + | |
| - | [[Category: Hansenula polymorpha]]
| + | |
| - | [[Category: Peroxisome import]]
| + | |
| - | [[Category: Pex22]]
| + | |
| - | [[Category: Pex4]]
| + | |
| - | [[Category: Transferase]]
| + | |
| - | [[Category: Ubiquitin conjugating enzyme]]
| + | |
| Structural highlights
Function
UBCX_PICAN Catalyzes the covalent attachment of ubiquitin to other proteins. Essential for peroxisome biogenesis. Required for UBC4-independent ubiquitination of PEX5.[PROSITE-ProRule:PRU00388]
Publication Abstract from PubMed
Pex4p is a peroxisomal E2 involved in ubiquitinating the conserved cysteine residue of the cycling receptor protein Pex5p. Previously, we demonstrated that Pex4p from the yeast Saccharomyces cerevisiae binds directly to the peroxisomal membrane protein Pex22p and that this interaction is vital for receptor ubiquitination. In addition, Pex22p binding allows Pex4p to specifically produce lysine 48 linked ubiquitin chains in vitro through an unknown mechanism. This activity is likely to play a role in targeting peroxisomal proteins for proteasomal degradation. Here we present the crystal structures of Pex4p alone and in complex with Pex22p from the yeast Hansenula polymorpha. Comparison of the two structures demonstrates significant differences to the active site of Pex4p upon Pex22p binding while molecular dynamics simulations suggest that Pex22p binding facilitates active site remodelling of Pex4p through an allosteric mechanism. Taken together, our data provide insights into how Pex22p binding allows Pex4p to build K48-linked Ub chains.
Structural insights into K48-linked ubiquitin chain formation by the Pex4p-Pex22p complex.,Groves MR, Schroer CFE, Middleton AJ, Lunev S, Danda N, Ali AM, Marrink SJ, Williams C Biochem Biophys Res Commun. 2017 Dec 28. pii: S0006-291X(17)32552-4. doi:, 10.1016/j.bbrc.2017.12.150. PMID:29288668[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Groves MR, Schroer CFE, Middleton AJ, Lunev S, Danda N, Ali AM, Marrink SJ, Williams C. Structural insights into K48-linked ubiquitin chain formation by the Pex4p-Pex22p complex. Biochem Biophys Res Commun. 2017 Dec 28. pii: S0006-291X(17)32552-4. doi:, 10.1016/j.bbrc.2017.12.150. PMID:29288668 doi:http://dx.doi.org/10.1016/j.bbrc.2017.12.150
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