1oba
From Proteopedia
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'''MULTIMODULAR PNEUMOCOCCAL CELL WALL ENDOLYSIN FROM PHAGE CP-1 COMPLEXED WITH CHOLINE''' | '''MULTIMODULAR PNEUMOCOCCAL CELL WALL ENDOLYSIN FROM PHAGE CP-1 COMPLEXED WITH CHOLINE''' | ||
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[[Category: Menendez, M.]] | [[Category: Menendez, M.]] | ||
[[Category: Monterroso, B.]] | [[Category: Monterroso, B.]] | ||
| - | [[Category: | + | [[Category: Choline]] |
| - | [[Category: | + | [[Category: Lysozyme]] |
| - | [[Category: | + | [[Category: Multimodular]] |
| - | [[Category: | + | [[Category: Murein hydrolase]] |
| - | [[Category: | + | [[Category: Phage cp-1 lysin]] |
| - | [[Category: | + | [[Category: Pneumococcal cell wall degradation,hydrolase glycosidase,bacterilytic enzyme]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:37:36 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 00:37, 3 May 2008
MULTIMODULAR PNEUMOCOCCAL CELL WALL ENDOLYSIN FROM PHAGE CP-1 COMPLEXED WITH CHOLINE
Overview
Pneumococcal bacteriophage-encoded lysins are modular choline binding proteins that have been shown to act as enzymatic antimicrobial agents (enzybiotics) against streptococcal infections. Here we present the crystal structures of the free and choline bound states of the Cpl-1 lysin, encoded by the pneumococcal phage Cp-1. While the catalytic module displays an irregular (beta/alpha)(5)beta(3) barrel, the cell wall-anchoring module is formed by six similar choline binding repeats (ChBrs), arranged into two different structural regions: a left-handed superhelical domain configuring two choline binding sites, and a beta sheet domain that contributes in bringing together the whole structure. Crystallographic and site-directed mutagenesis studies allow us to propose a general catalytic mechanism for the whole glycoside hydrolase family 25. Our work provides the first complete structure of a member of the large family of choline binding proteins and reveals that ChBrs are versatile elements able to tune the evolution and specificity of the pneumococcal surface proteins.
About this Structure
1OBA is a Single protein structure of sequence from Bacteriophage cp-1. Full crystallographic information is available from OCA.
Reference
Structural basis for selective recognition of pneumococcal cell wall by modular endolysin from phage Cp-1., Hermoso JA, Monterroso B, Albert A, Galan B, Ahrazem O, Garcia P, Martinez-Ripoll M, Garcia JL, Menendez M, Structure. 2003 Oct;11(10):1239-49. PMID:14527392 Page seeded by OCA on Sat May 3 03:37:36 2008
Categories: Bacteriophage cp-1 | Lysozyme | Single protein | Albert, A. | Garcia, J L. | Garcia, P. | Hermoso, J A. | Martinez-Ripoll, M. | Menendez, M. | Monterroso, B. | Choline | Multimodular | Murein hydrolase | Phage cp-1 lysin | Pneumococcal cell wall degradation,hydrolase glycosidase,bacterilytic enzyme
